SARS-CoV-2 Spike S1(D614G) Protein [His] (DAGC210)

Recombinant SARS-CoV-2 Spike S1(D614G) Protein from HEK293 Cells [His]

Product Overview
A DNA sequence encoding the SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein (YP_009724390.1) (Val16-Arg685(D614G)) was expressed with a polyhistidine tag at the C-terminus.
Nature
Recombinant
Predicted N terminal
Val16
Tag/Conjugate
His
Molecular Weight
The recombinant SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.41 kDa.
Bio-activity
Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 Spike S1(D614G), the EC50 of SARS-CoV-2 Spike S1(D614G) is 350-650 ng/mL.
Endotoxin
< 1.0 EU per ug protein as determined by the LAL method.
Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 Spike Protein; SARS-CoV-2 S1
Procedure
None
Purity
>90 % as determined by SDS-PAGE.
Format
Lyophilized
Size
100ug
Buffer
Lyophilized from sterile PBS,pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
Keywords
SARS-CoV-2; coronavirus; SARS-CoV-2 Spike Protein; SARS-CoV-2 S1

Data Examples


SARS-CoV-2 S1 protein (D614G), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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