Anti-SARS-CoV-2 S1 Monoclonal antibody (CABT-CS033)

Specifications


Host Species
Humanized
Antibody Isotype
IgG
Clone
BIB114
Species Reactivity
SARS-CoV-2
Immunogen
SARS-CoV-2 S1
Conjugate
unconjugated

Applications


Application Notes
We recommend the following antibodies for sandwich immunoassay (Capture - Detection):
CABT-CS031 - CABT-CS033
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


ELISA plate was coated by SARS-CoV-2 S1 protein, 100 μL/cell at 5μg/ml. The indirect ELISA analysis was performed by loading 100 μL per well of the anti-SARS-CoV-2 S1 mAb (BIB114) at various concentrations. The plate was incubated for 1 hours at 37°C, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100μL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37°C, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.

Target


Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 spike 1; SARS-CoV-2 spike protein; SARS-CoV-2 S1

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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