Rabbit Anti-KLH Polyclonal antibody [AP]

Antibody Isotype

IgG

Immunogen

Full length protein: KLH.

Conjugate

Application Notes

ELISA: 1:5000-10000

General Notes

This product (Catalog No. DPATB-H82707) is a rabbit-derived IgG polyclonal antibody raised against n/a KLH. It has been affinity purified by protein A. Small peptides, proteins or haptens (<5 kda) are not very antigenic when injected into animals. Antigenicity of haptens can be increased tremendously if they are covalently conjugated to bigger proteins referred as carrier proteins. Most common carrier proteins are albumin (BSA), thyroglobulin, ovalbumin, and hemocyanin from invertebrates. Hemocyanin are blood/lymph proteins found in only two groups: the mollusks and the arthropods. Keyhole limpet hemocyanin (KLH) is particularly preferred carrier proteins due to its very large size and antigenicity in common host species. Any carrier protein-hapten conjugate typically induces antibody responses to the epitopes on the carrier protein and the hapten. Antibody response can be followed by ELISA using either the carrier protein or the free hapten or free hapten conjugated to another carrier protein. Availability of antibodies to KLH may serve as positive control for the presence of anti-KLH antibodies.
DPATB-H82707 is suitable for use in ELISA (1:5,000–1:10,000) application.
DPATB-H82707 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

keyhole limpet hemocyanin; KLH