The rat anti-KLH IgG ELISA developed by CD allows rapid and quantitative measurement of rat anti-KLH IgG levels in serum or plasma samples.
Contents of Kit
No.
Components
Size
Storage Conditions
1
KLH Coated 96-well Plate
12 × 8 wells
2-8°C
2
Enzyme Conjugate Reagent
11 ml
2-8°C
3
Reference Standard
1 vial (lyophilized)
Store < -20°C
4
20× Wash Solution
50 ml
2-8°C
5
Diluent
30 ml
2-8°C
6
TMB Reagent (One-Step)
11 ml
2-8°C
7
Stop Solution (1N HCl)
11 ml
2-8°C
Storage
The reference standard should be stored at or below –20°C. All other kit components should be stored at 4°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described.
General Description
Recent studies have demonstrated that suppression of anti-KLH (keyhole limpet hemocyanin) IgG levels by therapeutic agents provides a useful indicator of immunosupression.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y-axis against anti-KLH IgG concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.
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