Anti-KLH monoclonal antibody (DMAB15662)

Mouse anti-KLH monoclonal antibody for WB, IHC, ELISA Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
23C5.H4.B9
Species Reactivity
N/A
Immunogen
Keyhole Limpet Hemocyanin, KLH.
Conjugate
Unconjugated

Applications


Application Notes
ELISA (1:20000-1:100000); WB (1:2000-1:10000); Immunohistochemistry (1:1000-1:5000) & The optimal working dilution should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
KLH; Keyhole Limpet Hemocyanin; Hemocyanin 2 c chain; Hemocyanin 2c chain; Hemocyanin A chain; Hemocyanin B chain

Product Background


Antigen Description
KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.

Citations


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References


Smiga, M; Bielecki, M; et al. Anti-HmuY Antibodies Specifically Recognize Porphyromonas gingivalis HmuY Protein but Not Homologous Proteins in Other Periodontopathogens. PLOS ONE 10:-(2015).
Manzano-Roman, R; Diaz-Martin, V; et al. Identification of protective linear B-cell epitopes on the subolesin/akirin orthologues of Ornithodoros spp. soft ticks. VACCINE 33:1046-1055(2015).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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