Anti-KLH monoclonal antibody (DMAB15662)
Mouse anti-KLH monoclonal antibody for WB, IHC, ELISA
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Keyhole Limpet Hemocyanin, KLH.
ELISA (1:20000-1:100000); WB (1:2000-1:10000); Immunohistochemistry (1:1000-1:5000) & The optimal working dilution should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
KLH; Keyhole Limpet Hemocyanin; Hemocyanin 2 c chain; Hemocyanin 2c chain; Hemocyanin A chain; Hemocyanin B chain
KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
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Smiga, M; Bielecki, M; et al. Anti-HmuY Antibodies Specifically Recognize Porphyromonas gingivalis HmuY Protein but Not Homologous Proteins in Other Periodontopathogens. PLOS ONE 10:-(2015).
Manzano-Roman, R; Diaz-Martin, V; et al. Identification of protective linear B-cell epitopes on the subolesin/akirin orthologues of Ornithodoros spp. soft ticks. VACCINE 33:1046-1055(2015).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.