This test kit allows rapid and quantitative measurement of anti-KLH IgG levels in serum or plasma.
Contents of Kit
1. Anti-KLH HRP stock Store ≤ -70°C 2. Anti-monkey IgG acridan stock Store ≤ -70°C 3. Monkey Anti-KLH IgG stock (2 vials) 4. KLH coated white plate (12 × 8-well) 5. Sample diluent; 50 ml 6. Conjugate diluent; 10 ml 7. 20× Wash solution; 50 ml 8. Trigger solution; 7 ml
Storage
Store the HRP and acridan conjugates at or below -70°C. The remainder of the kit should be stored at 2-8°C. The KLH coated white plate should be kept in a sealed bag with desiccant. The kit will remain stable for at least six months from the date of purchase, provided that the components are stored as described.
Standard Curve
A typical standard curve with sum of RLU values plotted on the Yaxis versus log10 anti-KLH IgG concentrations on the X-axis is shown on the following page. This curve is for illustration only and should not be used to calculate unknowns.
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