KLH IgM (Monkey) ELISA Kit

Sample

Serum, plasma

Species Reactivity

Monkey

Detection Method

Indirect-ELISA

Intended Use

This test kit allows rapid and quantitative measurement of anti-KLH IgM levels in serum or plasma.

Contents of Kit

Storage

On receipt, the anti-KLH IgM standard stock should be stored frozen at -20°C or lower. The remainder of the kit should be stored at 2-8°C, and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. DO NOT FREEZE THE HRP CONJUGATE OR TMB SOLUTIONS. Test kits will remain stable for six months from the date of purchase provided that the components are stored as described.

General Description

Drug candidates are routinely screened for evidence of immune system regulation during the discovery process. It is important that the immune response is not enhanced or diminished since such effects may lead to hypersensitivity or increased susceptibility to infection. Determination of a drug candidate's effects on anti-KLH (keyhole limpet hemocyanin) antibody levels allows easy assessment of immune system regulation. Animals are immunized with KLH while undergoing drug treatment, and serum is collected at appropriate times post immunization. Typically, serum collected 5-7 days after immunization is used for measurement of anti-KLH IgM levels, and serum collected 14+ days post immunization is used to measure anti-KLH IgG levels. Comparison of anti-KLH IgG or IgM levels in drug treated versus control groups reveals effects of the drug on the immune response.