Monkey anti-KLH IgG ELISA Kit

Sample

Serum, plasma

Species Reactivity

Monkey

Detection Method

iELISA

Intended Use

This test kit allows rapid and quantitative measurement of anti-KLH IgG levels in serum or plasma.

Contents of Kit

1. KLH coated 96-well plate (provided as 12 strips of 8 wells)
2. Anti Monkey IgG HRP Conjugate, 11 ml
3. Anti-KLH IgG Stock (lyophilized)^A
4. 20× Wash Solution: 50 ml
5. Diluent: 50 ml
6. TMB Reagent (One-Step): 11 ml
7. Stop Solution (1N HCl): 11 ml
^A The reference standard provided with the kit is of human origin and was calibrated using pure rhesus IgG. The use of human IgG as standard allows export of the kit without requirement for CITES documentation.

Storage

The test kit should be stored at 4°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. Test kits will remain stable for six months from the date of purchase.

General Description

Drug candidates are routinely screened for evidence of immune system regulation during the discovery process. It is important that the immune response is not enhanced or diminished since such effects may lead to hypersensitivity or increased susceptibility to infection. Determination of a drug candidate's effects on anti-KLH antibody levels allows easy assessment of immune system regulation.
Animals are immunized with KLH while undergoing drug treatment and serum is collected at appropriate times post immunization. Typically, serum collected 5-7 days after immunization is used for measurement of anti-KLH IgM levels, and serum collected 14+ days post immunization is used to measure anti-KLH IgG levels. Comparison of anti-KLH IgG or IgM levels in drug treated, versus control groups reveals effects on the immune response.

Standard Curve

A typical standard curve with optical density readings at 450nm on the Y-axis against anti-KLH IgG concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.