This kit allows quantitative measurement of human anti-KLH IgM levels in serum, plasma, and other fluids.
Contents of Kit
No.
Components
Size
Storage Conditions
1
KLH Coated 96-well plate
12 × 8 well strips
2-8°C
2
Anti-Human IgM HRP Conjugate
11 ml
2-8°C
3
Anti-KLH IgM Stock
1vial (lyophilized)
-20°C
4
20× Wash Solution
50 ml
2-8°C
5
Diluent
50 ml
2-8°C
6
TMB Reagent
11 ml
2-8°C
7
Stop Solution (1N HCl)
11 ml
2-8°C
Storage
The anti-KLH IgM stock should be stored at - 20°C or lower. The remainder of the kit should be stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air. The kit will remain stable for six months from the date of purchase provided that the components are stored as described.
Performance Characteristics
Parallelism: To assess performance of the assay, two samples containing anti-KLH IgM at concentrations of 58.5 and 8.7 μg/ml were serially diluted from 50- to 1600-fold to produce values within the dynamic range of the assay.
Standard Curve
A typical standard curve is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns.
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