The C44 hybridoma was originated by S.K. Edmond Rouan in 1987.Animals were immunized with a colchicine - keyhole limpet hemocyanin (KLH) conjugate. Spleen cells were fused with NS-1 myeloma cells. The antibody binds with high affinity to colchicine, and with lower affinity to related compounds. The antibody is bound strongly by Protein A.
Application Notes
It is monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
Have you cited CSC-H1124 in a publication? Let us know and earn a reward for your research.
My Review for Mouse Anti-Colchicine-keyhole Limpet Hemocyanin (KLH) Conjugate Hybridoma [D55]
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
