Anti-Aflatoxin monoclonal antibody, clone 3E8

In recent years, Vietnam is the leading country in exporting cashew to global market. However, tropical climate is a significantly favorable condition for fungus to develop when the cashew is not properly stored. For this reason, mycotoxin contaminations are likely to occur on the cashew, especially aflatoxin. By utilizing solid phase extraction technique with Oasis HLB stationary phase and optimizing conditions of sample preparation, the toxins such as aflatoxin B1, B2, G1 and G2 were extracted and concentrated from cashew samples. Simultaneously, with the assistance of ultra high-performance liquid chromatography coupled with high resolution mass spectrometry detector, this study has successfully fabricated concurrent analysis method of aflatoxin compounds with high sensitivity and selectivity. The method established in this study manifested a high linear range (R-2 >= 0.995), sensitivity (limits of detection ranged from 0.05 to 0.10 mu g/kg and limits of quantification varied from 0.5 to 1.0 mu g/kg), adequate recovery (96.5-109.4 %), and good repeatability (intra-day precision in the range of 2.2-6.1 % and inter-day precision in the range of 3.6-13.3 %). In particular, this method has been successfully applied for the determination of 4 aflatoxins in real cashew nut samples.

An efficient analytical method has been developed for the extraction and quantification of aflatoxin M(1)in milk samples using a modified air-assisted liquid-liquid microextraction method combined with high-performance liquid chromatography-fluorescence detector. Firstly, defatted milk is transferred into a glass test tube and its proteins are participated with zinc acetate solution. After vortexing and centrifugation of the mixture, the obtained supernatant is removed and transferred into a conical bottom glass test tube. Then, the adsorbed analyte onto the precipitated proteins is eluted with acetonitrile and after centrifugation, the eluant is mixed with the supernatant obtained from the previous step. A few microlitres of toluene (as an extraction solvent) is added into the solution and the mixture is sucked into a glass syringe and pushed out in the tube for four times. The obtained cloudy solution is centrifuged to collect the extraction solvent. Then, the lower aqueous phase is withdrawn by a glass syringe to transfer the extraction solvent to the conical section of the tube. The method was validated and under optimal conditions, it was found that the method has low limits of detection (0.9 ng L-1) and quantification (3 ng L-1), good linearity (r(2) = 0.9976), and satisfactory repeatability (relative standard deviation <= 9% for intra- (n = 8) and inter-day (n = 6) precisions). The extraction recovery and enrichment factor of AFM1 were 87% and 87, respectively. Finally, the proposed method was applied on different milk samples and concentration of aflatoxin M(1)was determined in the studied samples.