Sample
Serum or plasma (EDTA, citrate or heparin)
Intended Use
This product is used to detect whether neutralizing antibodies in a sample can competitively block the binding of viral proteins to host cell receptors.
Contents of Kit
1. Pre-coated Microplate 96 wells (12 strips of 8), 2-8°C
2. Sample Dilution Buffer, 2-8°C
3. HRP-conjugated receptor, 1 vial (lyophilized or liquid), -20°C (aliquot if liquid)
4. Conjugate Dilution Buffer, 2-8°C
5. Positive Control Serum, -20°C
6. Negative Control Serum, -20°C
7. Calibrator (or Internal Reference), -20°C
8. TMB Substrate Solution, 2-8°C, protect from light
9. Stop Solution, 2-8°C
10. 20× Wash Buffer Concentrate, 2-8°C
11. Plate Sealer, Room Temperature
Storage
The kit must be stored at 2 – 8°C. All components are stable until expiration date; please see a label on a kit box.
Citations
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Glasker, S; Lulla, A; et al. Virus replicon particle based Chikungunya virus neutralization assay using Gaussia luciferase as readout. VIROLOGY JOURNAL 10:-(2013).
Sheela, PJ; Sumathy, K; et al. Serological correlates of immune protection conferred by Chikungunya virus infection. ACTA VIROLOGICA 57:471-473(2013).