Monkey Anti-Chikungunya Virus E1 IgG ELISA Kit

Sample

Serum, Plasma, or other Biological Fluids

Species Reactivity

Monkey

Detection Method

iELISA

Intended Use

The Monkey Anti-Chikungunya Virus (CHIKV) E1 IgG ELISA Kit detects and quantifies anti-CHIKV E1 IgG in monkey serum or plasma of exposed or immunized animals. This immunoassay is suitable for:
Determining immune status relative to non-immune controls;
Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
Qualifying and/or standardizing vaccine batches and protocols.
This kit is for research use only (RUO), not for diagnosis or therapeutic purposes.

Contents of Kit

Wash Solution Concentrate (100×), 10ml
Sample Diluent Concentrate (20×), 10ml
Anti-Monkey IgG HRP Conjugate Concentrate (100×), 0.15ml
CHIKV E1 Coated Strip Plate, 8-well strips (12), Coated with Chikungunya E1 virus proteins; post-coated with stabilizers.
Anti-CHIKV E1 Calibrators, 1 U/ml, 2.5 U/ml, 5 U/ml, 10 U/ml, 0.65 ml each
Anti-CHIKV E1 Positive Control, 0.65ml, Anti-CHIKV E1 diluted in buffer with protein, detergents and antimicrobial.
Low NSB Sample Diluent (Reduces non-specific binding), 30 ml, Buffer with protein, detergents and antimicrobial.
TMB Substrate, 12 ml
Stop Solution, 12 ml

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label. Stabilities of the working solutions are indicated under Reagent Preparation.

General Description

Chikungunya virus (CHIKV) is an arthritogenic arbovirus belonging to the alphavirus genus of Togaviridae, transmitted to humans by infected female Aedes arthropods. CHIKV can cause acute infections with fever and severe joint pain; also chronic rheumatism. So far, CHIKV has been identified in over 60 countries in Europe, the Americas, Asia and Africa.
CHIKV is an enveloped, single-stranded positive-sense RNA virus, with a genome of about 11.7 kb including 5' and 3' ORFs. The 5' ORF is translated from genomic RNA by a cap-dependent mechanism resulting in the formation of 5 structural (envelope proteins E1-E3 forming trimeric spikes on the virions' surface, capsid and 6K/TF) and 4 non-structural proteins (nsP1-4).
While glycoprotein (GP) E1 is responsible for fusion within endosomes of target cells and nucleocapsid release, GP E2 interacts with cellular receptors for cell entry. The small GP E3 mediates pH-protection during virus biogenesis and prevents E1 from premature fusion. The capsid protein of alphavirus serves as a serine protease for self-cleavage, is necessary for the interaction with viral spike proteins during virion formation and serves a major function in nucleocapsid formation. The 6K/TF protein is essential for formation and budding of new virions.
Two-thirds of CHIK virus' RNA encodes for non-structural polyprotein precursors nsP1-4 serving RNA helicase, nucleoside triphosphatase and RNA dependent 5' triphosphatase enzymatic activities.
Vaccine development activities have focused primarily on the structural E1, E2, E3 glycoproteins; efforts have also included targeting the capsid, 6K and some non-structural proteins.