Chikungunya Virus IgM ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The ELISA Chikungunya Virus IgM tests are qualitative and quantitative immunoassay for the detection of human antibodies in serum or plasma directed against Chikungunya Virus.

Contents of Kit

1. Break apart microtiter test strips each with eight antigen coated single wells, (altogether 96), 1 frame. The coating material is inactivated.12 pieces
2. Standard serum (ready-to-use), Human serum in protein-containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface Antigen) and anti-HCV Ab; Preservative: <0.1% sodium azide; coloring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use), Human serum in protein-containing phosphate buffer; negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface Antigen) and anti-HCV Ab; Preservative: <0.1% sodium azide; coloring: Lissamin Green V. 2 ml
4. Anti-human IgM conjugate (ready-to-use), Anti-human IgM polyclonal antibody, Conjugated to alkaline phosphatase, stabilized with protein stabilization solution; Preservative: <0.1% methylisothiazolone, <0.1% bromnitrodioxane. 13ml
5. Washing solution concentrate (sufficient for 1000ml), Sodium chloride solution with Tween 20 and 30mM Tris-HCl, pH 7.4; Preservative: <0.1% sodium azide. 33.3ml
6. Dilution buffer (ready-to-use), Protein-containing phosphate buffer with Tween 20; Preservative: <0.1% sodium azide; coloring: 0.01g/l Bromphenol blue. 2 × 50ml
7. Stopping solution (ready-to-use), <0.1N sodium hydroxide, 40mM EDTA . 15ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate in solvent-free buffer; Preservative: <0.1% sodium azide. 13ml

Storage

General Description

Chikunguya Virus (CHIKV) is a member of the Alphavirus genus. It is also classified as an Arbovirus due to vector-dependent transmission by mosquitos. The enveloped virus carries structural glycoproteins E1-E3 which are immunogenic. Several non-structural proteins (nsP) and a ss-(+) RNA genome are present within the viral capsid.
Chikungunya fever is characterized by fever, joint pain and swelling, rash, and flu-like symptoms. Symptoms usually appear within a period of 1-2 weeks post-infection. The disease is characterized by a biphasic course with an initial stage of high virus load and a convalescent stage without viremia. In rare cases, neurological disorders like Guillain-Barré syndrome and meningoencephalitis have occurred.