Adenovirus IgM ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

This test is immunoassay for the detection of human antibodies in serum or plasma directed against all pathogenic human adenovirus serotypes.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame. The coating material is inactivated.
2. Cut-off serum (ready-to-use), 2 × 2ml. Human serum in protein-containing phosphate buffer: negative for anti HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab; preservative: <0.1% sodium azide; coloring: Chinaldin yellow
3. Positive control serum (ready-to-use), 2ml. Human serum in protein-containing phosphate buffer; negative for anti HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab; preservative: <0.1% sodium azide; coloring: Amaranth O
4. Negative control serum (ready-to-use), 2ml. Human serum in protein-containing phosphate buffer; negative for anti HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1% sodium azide; coloring: Lissamine green V
5. Anti-human-IgA, IgG, or IgM conjugate (ready-to-use), 13ml. Anti-human-IgA, IgG, or IgM polyclonal antibody, conjugated to alkaline phosphatase, stabilized with protein stabilization solution; preservative: 0.1% methylisothiazolone, <0.1 % bromnitrodioxane
6. Washing solution concentrate (sufficient for 1000ml), 33.3ml. Sodium chloride solution with Tween 20, 30mM Tris-HCl, pH 7.4; preservative: < 0.1% sodium azide
7. Dilution buffer (ready-to-use), 2 × 50ml. Protein-containing phosphate buffer with Tween 20; preservative: < 0.1% sodium azide; coloring: 0.01 g/l Bromphenol blue.
8. Stopping solution (ready-to-use), 15ml. <0.1N sodium hydroxide, 40mM EDTA
9. Substrate (ready-to-use), 13ml. Para-nitrophenylphosphate in solvent free buffer; preservative: < 0.1 % sodium azide

Storage

General Description

In the 1950s Adenoviruses were described as latent persisting agents in tonsils and adenoid tissue, as well as the cause of acute respiratory tract infection. Adenoviruses cause diseases in multiple organ systems (respiratory tract, gastrointestinal tract, eye- and urogenital tract).
Adenoviruses belong to the family of adenoviridae and are icosahedral particles with a size of 90-100nm. The virus consists of a protein capsid containing double-stranded DNA. Adenoviruses that are pathogenic for humans consist of 51 serotypes sorted into several subtypes. Serotype 1-8, 40, and 41 are most commonly isolated from humans.