Adenovirus IgM ELISA Kit

Name: Adenovirus IgM ELISA Kit
SKU: DEIA1767
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

serum, plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Adenovirus IgM ELISA is intended for the determination of IgM class antibodies against Adenovirus in human serum or plasma (citrate, heparin). For research use only-Not for use in diagnostic procedures.

Contents of Kit

1. Microtiterplate: 12 break-apart 8-well snap-off strips coated with Adenovirus antigens; in resealable aluminium foil.
2. IgM Sample Dilution Buffer: 1 bottle containing 100 mL of phosphate buffer (10 mM) for sample dilution; pH 7.2 ± 0.2; anti-human IgG (RF Absorbent); coloured green; ready to use; white cap; ≤ 0.0015% (v/v) CMIT/ MIT (3:1).
3. Stop Solution: 1 bottle containing 15 mL sulphuric acid, 0.2 mol/L; ready to use; red cap.
4. 20× Washing Buffer (20× conc.): 1 bottle containing 50 mL of a 20-fold concentrated phosphate buffer (0.2 M), pH 7.2 ± 0.2, for washing the wells; white cap.
5. Conjugate: 1 bottle containing 20 mL of peroxidase labelled antibody to human IgM in phosphate buffer (10 mM); coloured red; ready to use; black cap.
6. TMB Substrate Solution: 1 bottle containing 15 mL 3,3',5,5'-tetramethylbenzidine (TMB), < 0.1 %; ready to use; yellow cap.
7. Positive Control: 1 vial containing 2 mL control; coloured yellow; ready to use; red cap; ≤ 0.02% (v/v) MIT.
8. Cut-off Control: 1 vial containing 3 mL control; coloured yellow; ready to use; green cap; ≤ 0.02% (v/v) MIT.
9. Negative Control: 1 vial containing 2 mL control; coloured yellow; ready to use; blue cap; ≤ 0.0015% (v/v) CMIT/ MIT (3:1).
Controls are calibrated in arbitrary units against internal quality control samples, since no international standard reference is available for this assay.
For potential hazardous substances please check the safety data sheet.
10. Cover foil, 1
11. Instruction for use (IFU), 1
12. Plate layout,1

Storage

Store the kit at 2-8°C. The opened reagents are stable up to the expiry date stated on the label when stored at 2-8°C.

Precision

Sensitivity

The sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analyte. It is 90.0% (95% confidence interval: 68.3%-98.77%).

Citations

Publication (1)

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TGFβ links EBV to multisystem inflammatory syndrome in children

Goetzke, C. C., Massoud, M., Frischbutter, S., Guerra, G. M., Ferreira-Gomes, M., Heinrich, F., von Stuckrad, A. S. L., Wisniewski, S., Licha, J. R., Bondareva, M., Ehlers, L., Khaldi-Plassart, S., Javouhey, E., Pons, S., Trouillet-Assant, S., Ozsurekci, Y., Zhang, Y., Poli, M. C., Discepolo, V., Lo Vecchio, A., … Mashreghi, M. F.

Nature2025 AprPubMed ID: 40074901Read Article

Applications: ELISA

Reactive species: Human

"Abstract:In a subset of children and adolescents, SARS-CoV-2 infection induces a severe acute hyperinflammatory shock termed multisystem inflammatory syndrome in children (MIS-C) at four to eight weeks after infection. MIS-C is characterized by a specific T cell expansion and systemic hyperinflammation. The pathogenesis of MIS-C remains largely unknown. Here we show that acute MIS-C is characterized by impaired reactivation of virus-reactive memory T cells, which depends on increased serum levels of the cytokine TGFβ resembling those that occur during severe COVID-19. This functional impairment in T cell reactivity is accompanied by the presence of TGFβ-response signatures in T cells, B cells and monocytes along with reduced antigen-presentation capabilities of monocytes, and can be reversed by blocking TGFβ. Furthermore, T cell receptor repertoires of patients with MIS-C exhibit expansion of T cells expressing TCRVβ21.3, resembling Epstein-Barr virus (EBV)-reactive T cell clones capable of eliminating EBV-infected B cells. Additionally, serum TGFβ in patients with MIS-C can trigger EBV reactivation, which is reversible with TGFβ blockade. Clinically, the TGFβ-induced defect in T cell reactivity correlates with a higher EBV seroprevalence in patients with MIS-C compared with age-matched controls, along with the occurrence of EBV reactivation. Our findings establish a connection between SARS-CoV-2 infection and COVID-19 sequelae in children, in which impaired T cell cytotoxicity triggered by TGFβ overproduction leads to EBV reactivation and subsequent hyperinflammation."

"Article snippet:Serum IgG-antibody levels against HSV-1 (*), HSV2 (*), EBNA1 (*), CMV (*), HHV-6 (*) and AdV (Creative Diagnostics, DEIA2382) and serum IgM antibody levels against HSV-1/2 (*), EBNA1 (*), CMV (*), HHV-6 (Creative Diagnostics, DEIABL57) and AdV (Creative Diagnostics, DEIA1767) were measured in first serum samples obtained from patients according to manufacturer’s manuals."

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Datasheet

Certificate of Analysis

Safety Data Sheet

Customer Reviews

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Influence of Cu doping on the visible-light-induced photocatalytic activity of InVO4 (vol 7, pg 13911, 2017)

RSC ADVANCES

Authors: Wetchakun, Natda; Wanwaen, Pimonrat; Phanichphant, Sukon; Wetchakun, Khatcharin

Abstract

Correction for 'Influence of Cu doping on the visible-light-induced photocatalytic activity of InVO4' by Natda Wetchakun et al., RSC Adv., 2017, 7, 13911-13918, DOI: ; 10.1039/C6RA27138C.

Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission

VIRUSES-BASEL

Authors: Medkour, Hacene; Amona, Inestin; Akiana, Jean; Davoust, Bernard; Bitam, Idir; Levasseur, Anthony; Tall, Mamadou Lamine; Diatta, Georges; Sokhna, Cheikh; Adriana Hernandez-Aguilar, Raquel; Barciela, Amanda; Gorsane, Slim; La Scola, Bernard; Raoult, Didier; Fenollar, Florence; Mediannikov, Oleg

Abstract

Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The aim of this study was to investigate the occurrence and diversity of AdVs in: (i) fecal samples of apes and monkeys from different African countries (Republic of Congo, Senegal, Djibouti and Algeria), (ii) stool of humans living near gorillas in the Republic of Congo, in order to explore the potential zoonotic risks. Samples were screened by real-time and standard PCRs, followed by the sequencing of the partial DNA polymerase gene in order to identify the AdV species. The prevalence was 3.3 folds higher in NHPs than in humans. More than 1/3 (35.8%) of the NHPs and 1/10 (10.5%) of the humans excreted AdVs in their feces. The positive rate was high in great apes (46%), with a maximum of 54.2% in chimpanzees (Pan troglodytes) and 35.9% in gorillas (Gorilla gorilla), followed by monkeys (25.6%), with 27.5% in Barbary macaques (Macaca sylvanus) and 23.1% in baboons (sevenPapio papioand sixPapio hamadryas). No green monkeys (Chlorocebus sabaeus) were found to be positive for AdVs. The AdVs detected in NHPs were members ofHuman mastadenovirus E(HAdV-E), HAdV-C or HAdV-B, and those in the humans belonged to HAdV-C or HAdV-D. HAdV-C members were detected in both gorillas and humans, with evidence of zoonotic transmission since phylogenetic analysis revealed that gorilla AdVs belonging to HAdV-C were genetically identical to strains detected in humans who had been living around gorillas, and, inversely, a HAdV-C member HAdV type was detected in gorillas. This confirms the gorilla-to-human transmission of adenovirus. which has been reported previously. In addition, HAdV-E members, the most often detected here, are widely distributed among NHP species regardless of their origin, i.e., HAdV-E members seem to lack host specificity. Virus isolation was successful from a human sample and the strain of the Mbo024 genome, of 35 kb, that was identified as belonging to HAdV-D, exhibited close identity to HAdV-D members for all genes. This study provides information on the AdVs that infect African NHPs and the human populations living nearby, with an evident zoonotic transmission. It is likely that AdVs crossed the species barrier between different NHP species (especially HAdV-E members), between NHPs and humans (especially HAdV-C), but also between humans, NHPs and other animal species.