HSV Grade 2

Name: HSV Grade 2
SKU: DAG-H10363
Rating: 5 (1 reviews)

HSV Grade 2, native virus

COA

SDS

Datasheet

Summary

Nature

Native

Tag/Conjugate

Unconjugated

Preservative

None

Storage

Store this antigen preparation frozen at -70 °C to -1 00 °C. Repeated freezing and thawing should be avoided

Introduction

Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as Human herpes virus 1 and 2 (HHV-1 and -2), are two members of the herpes virus family, Herpesviridae, that infect humans.[1] Both HSV-1 (which produces most cold sores) and HSV-2 (which produce

Antigen Description

Grade 2 antigen contains a high concentration of virus particles and components in tissue culture medium containing a low concentration of bovine serum. The preparation contains no preservative or detergent. Some residual host cellular material is present

Keywords

Herpes simplex virus 2; HSV-2; Human herpes virus 2; HSV2

Inactivation

HSV antigen is inactivated using gamma radiation. This procedure is effective primarily by damaging viral genetic material.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Cognitive impairment and infectious burden in the elderly

ARCHIVES OF GERONTOLOGY AND GERIATRICS

Authors: Strandberg, TE; Pitkala, KH; Linnavuori, K; Tilvis, RS

Abstract

Infectious agents have been suspected as contributing factors to dementia, especially in Alzheimer disease. We intended to test whether viral or bacterial seropositivity is associated with cognitive impairment among home-dwelling elderly. Viral burden (seropositivity for herpes simplex type 1 (HSV1), type 2 (HSV2), or cytomegalovirus (CMV), and bacterial burden (Chlamydia pneumoniae and Mycoplasma pneumoniae) were tested among 383 home-dwelling individuals with vascular disease (mainly coronary heart disease) in the ongoing DEBATE study (mean age 80 years). Mini-mental state examination (MMSE) and its changes were used to define cognitive impairment. At baseline, 0-1, 2, and 3 positive titers toward viruses were found in 48 (12.5 %), 229 (59.8 %), and 106 (27.7 %) individuals, respectively. MMSE points decreased with increasing viral burden (p = 0.03). At baseline, 58 individuals (15.1 %) had cognitive impairment (MMSE < 24 points) which after adjustments was significantly associated with seropositivity for 3 viruses (risk ratio 2.5, 95 % confidence interval 1.3 to 4.7). MMSE score decreased in 150 cases (43 %) during 12-month follow-up. After adjustment for MMSE score at baseline and with 0-1 seropositivities as reference (1.0), the risk ratios were 1.8 (95 % confidence interval 0.9 to 3.6) and 2.3 (95 % confidence interval. 1.1 to 5.0) for 2 and 3 seropositivities, respectively. No significant associations were observed between bacterial burden and cognition. Viral burden of herpesvirus and cytomegalovirus was associated with cognitive impairment in home-dwelling elderly. The association may offer a preventable cause of cognitive decline.

CHEMILUMINESCENT AND COLORIMETRIC DETECTION OF A FLUORESCEIN-LABELED PROBE AND A DIGOXIGENIN-LABELED PROBE AFTER A SINGLE HYBRIDIZATION STEP

MOLECULAR AND CELLULAR PROBES

Authors: CHENAL, V; GRIFFAIS, R

Abstract

The objective of the simple and fast method we describe is the simultaneous hybridization of two non-radioactive probes and their detection from the same blot, using two different systems. These two probes are synthesized by PCR: one is label led with fluorescein and the other with digoxigenin. The former is detected by chemiluminescence and the latter by colorimetry. We applied this rapid and simple method to the specific detection of multiplex polymerase chain reaction products. We used the human herpes simplex virus HSV1 and HSV2 PCR models studied in our laboratory.