Animals were immunized with HSV-1. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody is type specific for HSV-1 but does not neutralize the virus.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, every 3 - 4 days adjust cell density to 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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