Human Herpes Simplex Virus1 IgA (HSV-1 IgA) ELISA Test kit

Name: Human Herpes Simplex Virus1 IgA (HSV-1 IgA) ELISA Test kit
SKU: DEIA345
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

HSV-1 IgA ELISA kit is intended for the detection of IgA antibodies to herpes simplex virus 1 (HSV 1).

Contents of Kit

1. Microwell Strips: purified HSV 1 antigen coated wells. (12 x 8 wells)
2. Absorbent Solution: Black Cap. 1 vial (22 ml)
3. Washing Concentrate, 10 ×: White Cap. 1 bottle (100 ml)
4. TMB Chromogenic Substrate: Amber bottle. 1 vial (12 ml)
5. Enzyme Conjugate: Red color solution. 1 vial (12 ml)
6. Cut-off Calibrator: Yellow Cap. HSV 1 A Index = 1.0 1 vial (150 μl)
7. Negative Control: Range stated on label. Natural Cap. 1 vial (150 μl)
8. Positive Control: Range stated on label. Red Cap. 1 vial (150 μl)
9. Stop Solution: 2 N HCl. 1 vial (12 ml)

Storage

1. Store the kit at 2 - 8°C.
2. Always keep microwells tightly sealed in pouch with desiccants. We recommend you use up all wells within 4 weeks after initial opening of the pouch.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light during storage or usage.

Precision

The precision of the assay was evaluated by testing three different sera of eight replicates over a period of one week.
The intra-assay and inter-assay C.V. are summarized below:

Sensitivity

98%

General Description

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. The antibodies present to HSV 1 may be of the IgA, IgM and IgG isotypes. The physiological function of IgA and its clinical implication is still unclear. The Creative Diagnostics ELISA HSV 1 IgA is an accurate and sensitive serologic method to detect HSV 1 antibody IgA isotype.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Identification of an antiviral compound isolated fromPistacia lentiscus

ARCHIVES OF MICROBIOLOGY

Authors: Bouslama, Lamjed; Benzekri, Roudaina; Nsaibia, Siwar; Papetti, Adele; Limam, Ferid

Abstract

This study screened mastic gum (Pistacia lentiscusL.) for antiviral activity against herpes simplex virus type 2 (HSV-2), coxsackievirus type B3, and adenovirus type 5. The organs of this plant (leaves, stem, and seed) were macerated sequentially using solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and methanol). Only the methanol extract of stem exhibited significant activity against HSV-2. This extract showed anti-HSV-2 activity with a selectivity index of 51 (50% cytotoxic concentration = 186 mu g/mL; 50% inhibitory concentration = 3.63 mu g/mL), and demonstrated direct inhibition against this virus with a virucidal selectivity index of 620 (50% virucidal concentration = 0.30 mu g/mL). A bio-guided assay involving thin-layer chromatography led to the isolation of two active compounds, which have been identified as dammaradienone and dammaradienol using high-performance liquid chromatography-diode array detection coupled with electrospray ionization mass spectrometry.P. lentiscushas been widely studied for other biological activities. However, to our knowledge, this is the first report ofP. lentiscusL. exhibiting antiviral activity.

BX795 demonstrates potent antiviral benefits against herpes simplex Virus-1 infection of human cell lines

ANTIVIRAL RESEARCH

Authors: Iqbal, Aqsa; Suryawanshi, Rahul; Yadavalli, Tejabhiram; Volety, Ipsita; Shukla, Deepak

Abstract

Herpes simplex virus-1 (HSV-1) infection is known to cause skin blisters, keratitis as well as deadly cases of encephalitis in some situations. Only a few therapeutic modalities are available for this globally prevalent infection. Very recently, a small molecule BX795 was identified as an inhibitor of HSV-1 protein synthesis in an ocular model of infection. In order to demonstrate its broader antiviral benefits, this study was aimed at evaluating the antiviral efficacy, mode-of-action, and toxicity of BX795 against HSV-1 infection of three human cell lines: HeLa, HEK, and HCE. Several different assays, including cell survival analysis, imaging, plaque analysis, Immunoblotting, and qRT-PCR, were performed. In all cases, BX795 demonstrated low toxicity at therapeutic concentration and showed strong antiviral benefits. Quite interestingly, cell line-dependent differences in the mechanism of antiviral action and cytokine response to infection were seen upon BX795 treatment. Taken together, our results suggest that BX795 may exert its antiviral benefits via cell-line specific mechanisms.