GeneralInformation
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with HSV-1. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody is type specific for HSV-1 but does not neutralize the virus.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, every 3 - 4 days adjust cell density to 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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Matundan, HH; Mott, KR; et al. Mutations within the Pathogenic Region of Herpes Simplex Virus 1 gK Signal Sequences Alter Cell Surface Expression and Neurovirulence. JOURNAL OF VIROLOGY 89:2530-2542(2015).
Priengprom, T; Ekalaksananan, T; et al. Synergistic effects of acyclovir and 3, 19-isopropylideneandrographolide on herpes simplex virus wild types and drug-resistant strains. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 15:-(2015).
COA
Certificate of Analysis
