SARS-CoV-2 Nucleocapsid (R203K, G204R) Protein [His] (DAGC515)

Recombinant SARS-CoV-2 Nucleocapsid (R203K, G204R) Protein from E. coli [His]

Target
SARS-CoV-2 NP
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (R203K, G204R)-His Recombinant Protein consists of 425 amino acids and predicts a molecular mass of 46.5 kDa.
Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 N Protein
Procedure
None
Purity
> 90 % as determined by SDS-PAGE.
Format
Lyophilized
Size
100 μg
Buffer
Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.0.
Preservative
None
Storage
Store it under sterile conditions at -20oC to -80oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Keywords
SARS-CoV-2; coronavirus; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 N Protein

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