Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody (CABT-CS027)

Mouse Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody for ELISA, WB, IHC

Specifications


Host Species
Mouse
Antibody Isotype
IgG
Clone
4B21
Species Reactivity
SARS-CoV-2, SARS
Immunogen
synthetic peptide PSDSTGSNQNGERSGARSKQ corresponding to a.a.20-39 of SARS-CoV-2 N protein.
Conjugate
unconjugated

Applications


Application Notes
Indirect ELISA: to be used at 0.05-1ug/mL.
Western Blot: to be used at 0.5-2ug/mL
Immunohistochemistry: to be used at 0.5-2ug/mL.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid Protein; 2019 Novel Coronavirus

Citations


Have you cited CABT-CS027 in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Acute SARS-CoV-2 Infection Impairs Dendritic Cell and T Cell Responses

IMMUNITY

Authors: Zhou, Runhong; To, Kelvin Kai-Wang; Wong, Yik-Chun; Liu, Li; Zhou, Biao; Li, Xin; Huang, Haode; Mo, Yufei; Luk, Tsz-Yat; Lau, Thomas Tsz-Kan; Yeung, Pauline; Chan, Wai-Ming; Wu, Alan Ka-Lun; Lung, Kwok-Cheung; Tsang, Owen Tak-Yin; Leung, Wai-Shing; Hung, Ivan Fan-Ngai; Yuen, Kwok-Yung; Chen, Zhiwei

The SARS-CoV-2 pandemic has resulted in millions of infections, yet the role of host immune responses in early COVID-19 pathogenesis remains unclear, By investigating 17 acute and 24 convalescent patients, we found that acute SARS-CoV-2 infection resulted in broad immune cell reduction including T, natural killer, monocyte, and dendritic cells (DCs). DCs were significantly reduced with functional impairment, and ratios of conventional DCs to plasmacytoid DCs were increased among acute severe patients. Besides lymphocytopenia, although neutralizing antibodies were rapidly and abundantly generated in patients, there were delayed receptor binding domain (RBD)- and nucleocapsid protein (NP)-specific T cell responses during the first 3 weeks after symptoms onset. Moreover, acute RBD- and NP-specific T cell responses included relatively more CD4 T cells than CD8 T cells. Our findings provided evidence that impaired DCs, together with timely inverted strong antibody but weak CD8 T cell responses, could contribute to acute COVID-19 pathogenesis and have implications for vaccine development.

Detection of SARS-CoV-2 by bronchoscopy after negative testing: Stay vigilant for COVID-19

RESPIRATORY MEDICINE CASE REPORTS

Authors: Ramos, Kathleen J.; Kapnadak, Siddhartha G.; Collins, Bridget F.; Pottinger, Paul S.; Wall, Richard; Mays, James A.; Perchetti, Garrett A.; Jerome, Keith R.; Khot, Sandeep; Limaye, Ajit P.; Mathias, Patrick C.; Greninger, Alexander

Purpose: Real-time polymerase chain reaction (RT-PCR) detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is required for diagnosis of coronavirus disease 2019 (COVID-19). Sensitivity of RT-PCR nasopharyngeal (NP) testing is presumed to be high, but there is no gold standard against which this has been determined. The objective was to determine whether lower respiratory tract infection (LRTI), detected in bronchoalveolar lavage fluid (BALF), occurs in the absence of upper respiratory tract infection with clinical testing of both specimen types. Methods: Between March 26, 2020 and April 17, 2020 at the University of Washington Medical Center all patients with BALF specimens clinically tested for SARS-CoV-2 were identified. We assessed the proportion of patients with positive RT-PCR for SARS-CoV-2 in BALF after negative NP testing. We describe 3 cases with positive testing in BALF. Results: Among 16 patients with BALF samples, 3 cases (19%) had SARS-CoV-2 detected in BALF. In Case 1, negative NP testing occurred early in the infection and respiratory symptoms may have been missed due to neurologic injury. In Case 2, outpatient diagnosis was aspiration pneumonia, but clinical suspicion remained high for COVID-19 at hospitalization based on epidemiological and clinical features. All 3 cases involved older adults (age >65 years), one of whom was immunosuppressed in the setting of lung transplantation (Case 3). Conclusions: These data demonstrate that SARS-CoV-2 LRTI occurs in the presence of negative NP testing. NP testing may underestimate the prevalence of COVID-19 and has implications for spread of SARS-CoV2 in the community and healthcare setting.

Skok, Kristijan, et al. "Post-mortem viral dynamics and tropism in COVID-19 patients in correlation with organ damage." Virchows Archiv 478.2 (2021): 343-353.

Have you cited CABT-CS027 in a publication? Let us know and earn a reward for your research.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket