Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody (CABT-CS037)

Human Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody for ELISA

Specifications


Host Species
Human
Antibody Isotype
IgG
Clone
CO10
Species Reactivity
SARS-CoV-2
Immunogen
SARS-CoV-2 NP
Conjugate
unconjugated

Data Examples


ELISA plate was coated by N protein, 100 µL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 µL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
ELISA plate was coated by SARS-CoV-2 NP, 100 µL/cell at 5µg/ml. The indirect ELISA analysis was performed by loading 100 µL per well of the anti- SARS-CoV-2 N mAb (CO10) at various concentrations. The plate was incubated for 1 hours at 37°C, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100µL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37°C, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.

Target


Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid Protein

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References


Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens

JCI INSIGHT

Authors: Liu, Jun; Babka, April M.; Kearney, Brian J.; Radoshitzky, Sheli R.; Kuhn, Jens H.; Zeng, Xiankun

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China, in December 2019. The virus rapidly spread globally, resulting in a public health crisis including almost 5 million cases and 323,256 deaths as of May 21, 2020. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected FFPE cell pellets. We identified a suitable rabbit polyclonal anti-SARS-CoV spike protein antibody and a mouse monoclonal anti-SARS-CoV nucleocapsid protein (NP) antibody for cross-detection of the respective SARS-CoV-2 proteins by IHC and immunofluorescence assay (IFA). Next, we established RNAscope in situ hybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex FISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. It is hoped that these reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models.

Evaluation of a novel antigen-based rapid detection test for the diagnosis of SARS-CoV-2 in respiratory samples

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES

Authors: Porte, Lorena; Legarraga, Paulette; Vollrath, Valeska; Aguilera, Ximena; Munita, Jose M.; Araos, Rafael; Pizarro, Gabriel; Vial, Pablo; Iruretagoyena, Mirentxu; Dittrich, Sabine; Weitzel, Thomas

Objectives: In the context of the coronavirus disease 2019 (COVID-19) pandemic, the development and validation of rapid and easy-to-perform diagnostic methods are of high priority. This study was performed to evaluate a novel rapid antigen detection test (RDT) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory samples. Methods: The fluorescence immunochromatographic SARS-CoV-2 antigen test (Bioeasy Biotechnology Co., Shenzhen, China) was evaluated using universal transport medium with nasopharyngeal (NP) and oropharyngeal (OP) swabs from suspected COVID-19 cases. Diagnostic accuracy was determined in comparison to SARS-CoV-2 real-time (RT)-PCR. Results: A total of 127 samples were included; 82 were RT-PCR-positive. The median patient age was 38 years, 53.5% were male, and 93.7% were from the first week after symptom onset. Overall sensitivity and specificity were 93.9% (95% confidence interval 86.5-97.4%) and 100% (95% confidence interval 92.1-100%), respectively, with a diagnostic accuracy of 96.1% and Kappa coefficient of 0.9. Sensitivity was significantly higher in samples with high viral loads. Conclusions: The RDT evaluated in this study showed a high sensitivity and specificity in samples mainly obtained during the first week of symptoms and with high viral loads, despite the use of a non-validated sample material. The assay has the potential to become an important tool for early diagnosis of SARS-CoV-2, particularly in situations with limited access to molecular methods. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

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