Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody (CABT-CS026)

Mouse Anti-SARS-CoV-2 Nucleoprotein Monoclonal antibody for ELISA, WB


Host Species
Antibody Isotype
Species Reactivity
synthetic peptide (PKKDKKKKADETQALPQRQKK a.a.368-388) of SARS-CoV-2 N protein.


Application Notes
Indirect ELISA: to be used at 0.05-1ug/mL.
Western Blot: to be used at 0.1-1ug/mL
Sandwich ELISA: to be used at 0.25-1ug/mL as detecting antibody
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.


Alternative Names
SARS-CoV-2; coronavirus; SARS-CoV-2 NP; SARS-CoV-2 Nucleocapsid Protein; 2019 Novel Coronavirus


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Acute SARS-CoV-2 Infection Impairs Dendritic Cell and T Cell Responses


Authors: Zhou, Runhong; To, Kelvin Kai-Wang; Wong, Yik-Chun; Liu, Li; Zhou, Biao; Li, Xin; Huang, Haode; Mo, Yufei; Luk, Tsz-Yat; Lau, Thomas Tsz-Kan; Yeung, Pauline; Chan, Wai-Ming; Wu, Alan Ka-Lun; Lung, Kwok-Cheung; Tsang, Owen Tak-Yin; Leung, Wai-Shing; Hung, Ivan Fan-Ngai; Yuen, Kwok-Yung; Chen, Zhiwei

The SARS-CoV-2 pandemic has resulted in millions of infections, yet the role of host immune responses in early COVID-19 pathogenesis remains unclear, By investigating 17 acute and 24 convalescent patients, we found that acute SARS-CoV-2 infection resulted in broad immune cell reduction including T, natural killer, monocyte, and dendritic cells (DCs). DCs were significantly reduced with functional impairment, and ratios of conventional DCs to plasmacytoid DCs were increased among acute severe patients. Besides lymphocytopenia, although neutralizing antibodies were rapidly and abundantly generated in patients, there were delayed receptor binding domain (RBD)- and nucleocapsid protein (NP)-specific T cell responses during the first 3 weeks after symptoms onset. Moreover, acute RBD- and NP-specific T cell responses included relatively more CD4 T cells than CD8 T cells. Our findings provided evidence that impaired DCs, together with timely inverted strong antibody but weak CD8 T cell responses, could contribute to acute COVID-19 pathogenesis and have implications for vaccine development.

Evaluation of a novel antigen-based rapid detection test for the diagnosis of SARS-CoV-2 in respiratory samples


Authors: Porte, Lorena; Legarraga, Paulette; Vollrath, Valeska; Aguilera, Ximena; Munita, Jose M.; Araos, Rafael; Pizarro, Gabriel; Vial, Pablo; Iruretagoyena, Mirentxu; Dittrich, Sabine; Weitzel, Thomas

Objectives: In the context of the coronavirus disease 2019 (COVID-19) pandemic, the development and validation of rapid and easy-to-perform diagnostic methods are of high priority. This study was performed to evaluate a novel rapid antigen detection test (RDT) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory samples. Methods: The fluorescence immunochromatographic SARS-CoV-2 antigen test (Bioeasy Biotechnology Co., Shenzhen, China) was evaluated using universal transport medium with nasopharyngeal (NP) and oropharyngeal (OP) swabs from suspected COVID-19 cases. Diagnostic accuracy was determined in comparison to SARS-CoV-2 real-time (RT)-PCR. Results: A total of 127 samples were included; 82 were RT-PCR-positive. The median patient age was 38 years, 53.5% were male, and 93.7% were from the first week after symptom onset. Overall sensitivity and specificity were 93.9% (95% confidence interval 86.5-97.4%) and 100% (95% confidence interval 92.1-100%), respectively, with a diagnostic accuracy of 96.1% and Kappa coefficient of 0.9. Sensitivity was significantly higher in samples with high viral loads. Conclusions: The RDT evaluated in this study showed a high sensitivity and specificity in samples mainly obtained during the first week of symptoms and with high viral loads, despite the use of a non-validated sample material. The assay has the potential to become an important tool for early diagnosis of SARS-CoV-2, particularly in situations with limited access to molecular methods. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

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