COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA Kit

Name: COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA Kit
SKU: DEIA-SY2335
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Cell Culture Supernatants, Serum, Nasopharyngeal swab

Detection Method

sELISA

Intended Use

The COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID 19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.

Contents of Kit

No.	Components	Size	Storage Conditions
1	COVID-19 N Protein Microplate	96T	See Datasheet
2	COVID-19 N Protein Standard Protein	2 vials	See Datasheet
3	COVID-19 N Protein Detection Antibody	2 vials	See Datasheet
4	Wash Buffer (20×)	25 ml	See Datasheet
5	HRP-Streptavidin (100×)	200 μl	See Datasheet
6	TMB One-Step Substrate Reagent	12 ml	See Datasheet
7	Stop Solution	8 ml	See Datasheet
8	Assay Diluent B (5×)	15 ml	See Datasheet

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see 'Reagents And Materials Provided'.

Detection Range

0.07 ng/ml - 50 ng/ml

Sensitivity

The minimum detectable dose of COVID-19 N Protein was determined to be 0.07 ng/ml.
Minimum detectable dose is defined as the analyte concentration resulting in an absorbance that is 2 standard deviations higher than that of the blank (diluent buffer).

General Description

The spike protein (S-protein) and nucleocapsid protein (N-protein) are encoded by all coronaviruses, including the coronavirus (COVID-19). Nucleocapsid protein is a most abundant protein of coronavirus. The nucleocapsid protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N protein of coronavirus is chosen as a diagnostic tool since its strong immunogenicity.

Citations