Sample
serum, cell culture supernatants
Intended Use
The COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum(plasma is not recommended in this assay) and cell culture supernatants.
Contents of Kit
1. N-Protein Microplate*: 1 Plate, 96 wells (12 × 8) coated with anti-N-Protein. Storage / Stability After Preparation: 1 month at 4°C
2. N-Protein Standard Protein: 2 vials, Each vial is enough to run each standard in duplicate. Storage / Stability After Preparation: 1 week at -80°C
3. N-Protein Detection Antibody: 2 vials, Each vial is enough to assay half the microplate. Storage / Stability After Preparation: 5 days at 4°C
4. Wash Buffer (20×): 25 mL, 20× concentrated solution. Storage / Stability After Preparation: 1 month at 4°C
5. HRP-StreptavidinL: 200 μL, 100× concentrated HRP-conjugated streptavidin. Do not store and reuse
6. TMB One-Step Substrate Reagent: 12 mL, 3,3,5,5'-tetramethylbenzidine in buffer solution.
7. Stop Solution: 8 mL, 0.2 M sulfuric acid
8. Assay Diluent B (5×): 15 mL. 5× concentrated diluent buffer. Storage / Stability After Preparation: 1 month at 4°C
9. 96-Well Plate Sealing Film**: 2 films. Microplate adhesive sealing film
*Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
**Optional use for overnight incubations.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
Precision
Intra-Assay CV%: <10%
Inter-Assay CV%: <12%
Sensitivity
The minimum detectable dose of N-Protein was determined to be 0.07 ng/ml.
Minimum detectable dose is defined as the analyte concentration resulting in an absorbance that is 2 standard deviations higher than that of the blank (diluent buffer).
General Description
The spike protein (S-protein) and nucleocapsid protein (N-protein) are encoded by all coronaviruses, including the coronavirus (COVID-19). Nucleocapsid protein is a most abundant protein of coronavirus. The nucleocapsid protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N protein of coronavirus is chosen as a diagnostic tool since its strong immunogenicity.
Standard Curve
These standard curves are for demonstration only. A standard curve must be run with each assay.
Citations
Publication ()
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COA
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