Molecular Weight
50.4 kDa
Alternative Names
Lipase 1; Glycerol ester hydrolase 1; lip1; SACOL2694
Purity
> 90% as determined by SDS-PAGE
Format
Liquid or Lyophilized powder
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Introduction
Staphylococcus aureus Lipase (Geh) is a secreted enzyme that hydrolyzes triglycerides into free fatty acids, facilitating bacterial colonization on lipid-rich human skin and nutrient acquisition. Processed by the protease aureolysin (Aur), mature Geh enables S. aureus to degrade antimicrobial lipids in the skin, evading innate immune defenses. It enhances biofilm formation by integrating into the extracellular matrix and contributes to abscess development in deep tissues. Geh also synergizes with proteases (e.g., Aur) to inactivate host antimicrobial peptides, promoting systemic infections like bacteremia. Antibodies targeting Geh reduce biofilm biomass and show promise as therapeutic agents or vaccine components.
Keywords
Staphylococcus aureus Lipase 1; Glycerol ester hydrolase 1; lip1; SACOL2694; EC:3.1.1.3
Citations
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Varshney, AK; Wang, XB; et al. Isotype Switching Increases Efficacy of Antibody Protection against Staphylococcal Enterotoxin B-Induced Lethal Shock and Staphylococcus aureus Sepsis in Mice. MBIO 5:-(2014).
Devora, GA; Sun, LJ; et al. A Novel Missense Mutation in the Nuclear Factor-kappa B Essential Modulator (NEMO) Gene Resulting in Impaired Activation of the NF-kappa B Pathway and a Unique Clinical Phenotype Presenting as MRSA Subdural Empyema. JOURNAL OF CLINICAL IMMUNOLOGY 30:881-885(2010).
COA
Certificate of Analysis
