Product Overview
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Molecular Weight
53.5 kDa
Alternative Names
Replication initiation protein; repD
Purity
> 90% as determined by SDS-PAGE
Format
Liquid or Lyophilized powder
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Introduction
Replication initiation protein (RepD) is a 53.5 kDa plasmid-encoded protein (311 amino acids) in Staphylococcus aureus that functions as a specific topoisomerase to initiate plasmid replication. It binds plasmid DNA origin sequences and catalyzes strand cleavage/religation, enabling replication loop formation. RepD is rate-limiting for plasmid propagation in vivo and essential for mobilizing antibiotic resistance genes. Recombinant RepD (full-length, N-terminal 6×His-SUMO-tagged) is expressed in E. coli with >90% purity for studying plasmid dynamics and horizontal gene transfer mechanisms.
Keywords
Staphylococcus aureus Replication initiation protein; repD
Citations
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