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References
The Prevalence of Human Parainfluenza Virus 1 on Indoor Office Fomites
The objective of this study was to evaluate the potential role of fomites in human parainfluenza virus 1 (HPIV1) transmission by assessing the occurrence of HPIV1 on surfaces in an adult setting (office). In 2004, a total of 328 fomites from 12 different office buildings in five different cities were evaluated for HPIV1 viral RNA. HPIV1 was isolated using reverse transcriptase-polymerase chain reaction (RT-PCR) and detected on 37% of all office fomites. HPIV1 RNA was frequently isolated on desk tops (47%), and infrequently isolated on light switches (19%). Data revealed a statistically significant difference between the percentage of HPIV1 positive fomites in office cubicles and conference rooms (Chi-square P < 0.011, Fisher's Exact P = 0.054). A statistically significant difference was also found among positive fomites in different buildings (Chi-square P < 0.011). HPIV1 was consistently isolated on various indoor fomites in the 12 office buildings assessed during 2004, a low HPIV incident year.
Application of the digital shearing method to extract three-component velocity in holographic particle image velocimetry
We have recently proposed a new method to extract the three-dimensional (3D) velocity vector data from double-exposure holographic particle image velocimetry (HPIV), which we call the digital shearing method. In contrast to the full 3D correlation, it has been shown that all three components (3Cs) of particle image displacement can be retrieved using six two-dimensional fast Fourier transform operations and appropriate coordinate transformations. In this paper we demonstrate the capabilities of this approach on actual HPIV data. The holographic recording method described uses an imaging system to record a hologram of high numerical aperture using a conventional 35 mm film. The holograms are digitized and particle images are reconstructed numerically. From particle images reconstructed from separate holograms, we illustrate the analysis process by computing the 3Cs of particle image displacement in a step-by-step manner.