Anti-HBV Surface Antigen Monoclonal antibody, Clone JD24

Immature immune system and immune tolerance induced by exposure to HBeAg in utero and/or shortly after infection in newborns were reportedly the causes of chronic HBV infection. To investigate the effect of maternal-derived HBeAg on neonatal T cell immunity, we analysed and compared T cell phenotypes and functions among neonates born to HBsAg(+)/HBeAg(+)mothers (HBeAg(+)neonates), HBsAg(+)/HBeAg(-)mothers (HBeAg(-)neonates) and healthy control mothers (HC neonates), using flow cytometry. The results showed that neonatal T cell phenotypes were similar regardless of HBeAg exposure. Upon anti-CD3 and anti-CD28 stimulation in HBeAg(+)neonates, CD4(+)T cell production of IFN-gamma (P < .05) was significantly enhanced, while CD8(+)T cells secreted significantly more IL-2 compared with those in HBeAg(-)and HC groups (P < .05). Moreover, similar levels of IFN-gamma and IL-10 were observed in the culture supernatant after stimulation with rHBsAg, rHBcAg or rHBeAg among HBeAg+, HBeAg(-)and HC neonates, whereas HBeAg(+)neonates produced more TNF-alpha than HBeAg(-)neonates upon stimulation with rHBcAg. In conclusion, the results indicated that the HBsAg(+)/HBeAg(+)maternal environment did not influence the phenotypes of cord blood T cells but boosted neonatal non-specific Th1-type cytokine production.

Objective: National Health Commission of the People's Republic of China collaborated with many ministries and commissions government and initiated a population-based cancer screening program in high-risk area of rural China, targeting three types of cancer that are most prevalent in these areas, including esophageal, stomach and liver cancer. This study protocol was reported to show the design and evaluate the effectiveness of cancer screening and appropriate screening strategies of three cancers in rural China. Methods and analysis: A two-step design with cancer risk assessment based on questionnaire interview, Hepatitis B surface antigen (HBsAg) test strip and subsequent clinical intervention for high-risk populations was adopted free of charge at the local hospitals designated in the program. Ethic and dissemination: This study was approved by the Institutional Review Board of Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. The results will evaluate the effectiveness of cancer screening and appropriate screening strategies in rural China.