Mouse Anti-HBsAg IgG ELISA Kit (DEIASL263)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Species Reactivity
Intended Use
This kit is intended to detect anti-HBsAg IgG antibody in mouse serum post vaccination.
All reagents should be stored at 2-8°C , and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Indications of Deterioration
The Mouse Anti-HBsAg Assay may be considered to have deteriorated if:
1. The kit fails to meet the required criteria for a valid test (see Quality Control).
2. Reagents becoming visibly cloudy or develop precipitate. Note: Concentrated Wash buffer, when cold, normally develops crystalline precipitates, which re-dissolve on heating at 37°C.
3. The Substrate Solution turns dark green. This is likely to be caused by contamination of the Substrate Solution.
General Description
Hepatitis B virus can infect hominoidae and cause an inflammation of the liver called hepatitis. The virus is a hepadnavirus. This is a unique DNA virus as it replicates by a RNA intermediate using reverse transcription. During its life cycle the virus produces excess quantities of non-infectious particles designated as the hepatitis surface antigen (HBsAg). Animals will make antibodies to this antigen designating immunity. Vaccines have been developed with recombinant HBsAg to protect animals from infection. Antibodies should be detectable in animals post vaccination.


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Sugunan, AP; Bhattacharya, H; et al. Impact of hepatitis B immunization among the Nicobarese tribe - antibody titres & seroprotection five years after vaccination. INDIAN JOURNAL OF MEDICAL RESEARCH 139:427-430(2014).
Kim, E; Yune, S; et al. Hepatitis B Virus Reactivation During Anti-Cancer Chemotherapy in Patients with Past Hepatitis B Virus Infection. HEPATO-GASTROENTEROLOGY 61:1704-1711(2014).

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