Hepatitis B Surface Antigen (HBsAg) ELISA Kit

Name: Hepatitis B Surface Antigen (HBsAg) ELISA Kit
SKU: DEIA-NS2502-3
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Purified virus or unpurified viral supernatant

Detection Method

sELISA

Intended Use

Hepatitis B Surface Antigen (HBsAg) ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Hepatitis B Surface Antigen.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti-HBsAg Antibody Coated Plate	96T	4°C
2	FITC-Conjugated Anti-HBsAg Monoclonal Antibody	60 μL×1	4°C
3	HRP-Conjugated Anti-FITC Monoclonal Antibody	20 μL×1	4°C
4	Assay Diluent	50 mL×1	4°C
5	Triton X-100 Solution	15 mL×1	4°C
6	10X Wash Buffer	100 mL×1	4°C
7	Substrate Solution	12 mL×1	4°C
8	Stop Solution	12 mL×1	4°C
9	Recombinant HBsAg Standard	100 μL×1	-20°C

Storage

Upon receiving, aliquot and store recombinant HBsAg Standard at -20°C and avoid freeze/thaw. Store all other components at 4°C.

Detection Limit

1 ng/ml

General Description

Hepatitis B is an infection of the liver caused by the hepatitis B virus. HBV is transmitted by exposure to infectious blood or body fluids (e.g. saliva, semen, urine); forms of transmission include unprotected sexual activity, blood transfusion, mother-to-infant transmission, or consuming contaminated food/water. The acute illness causes liver inflammation, vomiting and jaundice, while chronic HBV infection often leads to liver cirrhosis and cancer.
Roughly one third of the world's population have been infected with hepatitis B virus. 5-10% of adults and 90% of babies who have been infected will have the virus for the rest of their lives. The infection is preventable by vaccination.
Diagnosis of chronic Hepatitis B Virus (HBV) infection has long been based on HBV serology and measurement of hepatocytic enzymes. With the development of therapies for chronic HBV infection, including interferon and lamivudine, quantitative detection of HBV (viral antigens and patient antibodies) has been used increasingly as the most important marker for monitoring HBV replication activity, disease progression, and assessing antiviral treatment. Specifically, Hepatitis B surface antigen (HBsAg) is an important diagnostic marker, generally detectable in patients with acute and chronic infection; positive testing indicates high HBV replication in the liver, elevated blood HBV titers and greater infectivity to others. Several assays for the quantitative measurement of HBV DNA have been developed, such as PCR-based nucleic acid amplification assays. However, these methods tend to be cumbersome and expensive.
Creative Diagnostics' Hepatitis B Surface Antigen (HBsAg) ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Hepatitis B Surface Antigen. The kit has detection sensitivity limit of ~1 ng/mL HBsAg. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HBsAg samples.

Standard Curve

The following figures demonstrate typical HBsAg ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.