HBsAg Glycan ELISA Kit

Sample

Cell culture supernatant, serum, and plasma

Species Reactivity

Human

Detection Method

ELISA

Intended Use

The HBsAg Glycan ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of HBV particles containing O-glycosylated M-HBsAg. For Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. HBsAgGi antibody coated plate: 96 well (8-well strip x 12)
2. Standard M-HBsAg (800 ng/mL): 1 mL (2 mL tube)
3. Dilution Buffer: 24 mL (12 mL x 2 bottles)
4. HRP-labeled HBsAgGi antibody: 11 mL
5. 20X Wash Buffer: 50 mL
6. TMB Substrate: 12 mL
7. Stop Solution (0.2 M Sulfuric acid): 12 mL
8. Plate seal: 3
9. User Guide

Storage

All kit components except Stop Solution must be stored at 2-8°C. All reagents are stable for 12 months after manufacturing when stored at the indicated conditions.

General Description

The HBs antigen that constitutes the envelope of HBV particles consists of three glycoproteins of different sizes (S-HBs antigen, M-HBs antigen, and L-HBs antigen), and infectious HBV particles with HBV DNA or HBV RNA have all HBs antigens. On the other hand, the blood of HBV-infected patients contains many subviral particles that are not infectious, and the subviral particles mainly consist of S-HBs antigen. The anti-HBV surface antigen glycosylated isomeric antibody (HBsAgGi) used in this kit is specific for genotype C and binds mainly to infectious HBV particles.

General Notes

The HBsAg Glycan ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of HBV particles containing O-glycosylated M-HBsAg. For Research Use Only. Not for use in diagnostic procedures.