Mouse Anti-HBsAg IgG ELISA Kit

Sample

Serum

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse Anti-HBsAg Assay is designed for quantitative analysis of HBsAg in samples.

Contents of Kit

1. Coated microwell strips. Plastic microtiter wells coated with recombinant HBsAg (adw and adr) in foil pouch with desiccant. 1 plate (96 wells)
2. Mouse Anti-HBsAg Positive Calibrator 10000 U/ml 0.4 mL
3. Sample Diluent 30 mL
4. Conjugate. Anti- mouse IgG conjugated to horseradish peroxidase enzyme containing 0.01% Bromonitrodioxane as preservative. 12 mL
5. Wash Buffer (20x concentrated). Tris buffered saline pH 7.8-8.0, containing 0.05% Tween 20. Must be diluted before use. 1 Bottles 60 mL
6. Substrate Solution. Ready to use. 2,2' Azino-di[3-ethyl-benzthiazolinesulfonate] ABTS 12 mL
7. Stop Solution. 1.25% sodium fluoride 5 mL

Storage

Store at 2-8°C (Sealed)
All reagents should be stored at 2-8°C , and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Indications of Deterioration
The Mouse Anti-HBsAg Assay may be considered to have deteriorated if:
1. The kit fails to meet the required criteria for a valid test.
2. Reagents becoming visibly cloudy or develop precipitate. Note: Concentrated Wash buffer, when cold, normally develops crystalline precipitates, which re-dissolve on heating at 37°C.
3. The Substrate Solution turns dark green. This is likely to be caused by contamination of the Substrate Solution.

General Description

Hepatitis B virus can infect hominoidae and cause an inflammation of the liver called hepatitis. The virus is a hepadnavirus. This is a unique DNA virus as it replicates by a RNA intermediate using reverse transcription. During its life cycle the virus produces excess quantities of non-infectious particles designated as the hepatitis surface antigen (HBsAg). Animals will make antibodies to this antigen designating immunity. Vaccines have been developed with recombinant HBsAg to protect animals from infection. Antibodies should be detectable in animals post vaccination.

General Notes

Hepatitis B virus can infect hominoidae and cause an inflammation of the liver called hepatitis. The virus is a hepadnavirus. This is a unique DNA virus as it replicates by a RNA intermediate using reverse transcription. During its life cycle the virus produces excess quantities of non-infectious particles designated as the hepatitis surface antigen (HBsAg). Animals will make antibodies to this antigen designating immunity. Vaccines have been developed with recombinant HBsAg to protect animals from infection. Antibodies should be detectable in animals post vaccination.
The Mouse Anti-HBsAg Assay is designed for quantitative analysis of HBsAg in samples.