The Mouse Anti-AAV8 ELISA is used as an analytical tool for quantitative detection of antibodies to AAV8 in Mouse serum.
Storage
1. All reagents should be stored at 2°C to 8°C for stability. 2. All the reagents and wash solutions should be used within 12 months from manufacturing date. 3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions. 4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Precision
Intra-Assay: CV＜15% Inter-Assay: CV＜15%
Detection Range
For mouse anti-AAV8 IgG in serum, the method has been demonstrated to be highly (>0.99) linear from 0 to 250 ng/mL.
Sensitivity
The Detection Threshold for the assay is 1.72 ng/mL. The assay sensitivity for undiluted samples corresponds to 34.4 ng/mL, because the serum samples are instructed to be diluted at 20-fold (1:20) before starting the assay.
Standard Curve
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