Anti-AAV2 (intact particle) monoclonal antibody

Adeno-associated viruses (AAV) are small, icosahedral viruses that have become one of the most important gene vectors in the field of gene therapy due to their long-term expression, low toxicity, low immunogenicity, and high tissue specificity. AAV2-based vectors are commonly used in gene therapy as they exhibit several desirable characteristics, including non-pathogenicity based on wild-type (wt) virus, the ability to infect dividing and non-dividing cells, and the establishment of long-term expression of heterologous genes by recombinant AAV. However, an important consideration in using this vector system is the high prevalence of anti-AAV-2 antibodies in humans. Many of these antibodies are neutralizing and may hinder the effectiveness of AAV2-based gene therapies. Several mechanisms of neutralization have been described, including interference with receptor attachment, inhibition of uncoating, induction of capsid structural changes, and interparticle cross-linking (aggregation).
Identification of neutralizing epitopes can aid in the development of less immunogenic vectors. The anti-AAV2 (intact particle) monoclonal antibody (CABT-B9062) is a valuable tool for characterizing different stages of AAV2 infection and analyzing the AAV assembly process. It specifically targets intact AAV2 particles, including both empty and full capsids. One key feature of this monoclonal antibody is its recognition of a conformational epitope found on assembled capsids. This epitope is not present in denatured capsid proteins or native but unassembled capsid proteins. This means that the antibody selectively binds to AAV2 particles that have undergone the proper assembly process, providing a reliable marker for fully assembled and functional capsids.
Moreover, the antibody's specificity for intact AAV2 particles makes it unsuitable for immunoblotting. This technique relies on the denaturation of proteins, which disrupts the conformational epitopes recognized by the Anti-AAV2 (intact particle) monoclonal antibody. Therefore, alternative methods such as immunofluorescence or ELISA are more appropriate for the detection and analysis of intact AAV2 particles using this antibody.

Anti-AAV2 (intact particle) monoclonal antibody
Anti-Adeno-associated virus type 2(intact particle) monoclonal antibody
Anti-Adeno-associated virus 2 (intact particle) monoclonal antibody