Species Reactivity
Monkey
Intended Use
The Anti-AAV6 ELISA is used as an analytical tool for qualitative determination of antibodies to AAV6 in serum.
Contents of Kit
1. AAV6 Capsid Coated Microtiter Plate, 12×8 wells
2. Negative Control, lyophilized
3. Anti-AAV6 Positive Control, lyophilized
4. Anti-IgG Conjugate, 100× , 1 × 0.12 ml
5. Sample Diluent, ready to use, 1 × 50 ml
6. Wash Buffer, 20 × concentrate, 1 × 50 ml
7. TMB Substrate, ready to use, 2 × 6 ml
8. Stop Solution, ready to use, 1 × 7 ml
9. Instruction Manual
Storage
1. All reagents should be stored at 2°C to 8°C for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25°C). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Standard Curve
Determine the Mean Absorbance for each set of duplicate control and Samples.
Calculate average (Avg) OD: Avg OD = Average OD from AAV6 Capsid Coated Microtiter Plate
Avg OD of the Monkey Anti-AAV6 Positive Control should be ≥ 0.3.
Avg OD of the Negative Control should be < 0.1.
CUT OFF value = Negative Control + 0.1.
Citations
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