Human C-Reactive Protein/CRP DuoSet ELISA Kit

Sample

Cell culture supernate, serum, and plasma samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the development of sandwich ELISAs to measure natural and recombinant CRP. The Reagent Diluent recommended may be suitable formost cell culture supernate, serum, and plasma samples. The Reagent Diluent selected for use can alter the performance of an immunoassay. Reagent Diluent optimization for samples with complex matrices such as serum and plasma, may improve their performance in this assay.
This kit contains sufficient materials to run ELISAs on 96 well plates, provided the following conditions are met:
1. The reagents are prepared as described in this package insert.
2. The assay is run as described in the General ELISA Protocol.
3. The recommended microplates, buffers, diluents, substrates, and solutions are used.
This package insert must be read in its entirety before using this product. Refer to the Certificate of Analysis for component concentrations as they may vary. For research use only. Not for use in diagnostic procedures.

Contents of Kit

1. Human C-Reactive Protein/CRP Capture Antibody
2. Human C-Reactive Protein/CRP Detection Antibody
3. Human C-Reactive Protein/CRP Standard
4. Streptavidin-HRP A

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Detection Range

15.6 - 1,000 pg/mL

Standard Curve

This standard curve is only for demonstration purposes. A standard curveshould be generated for each set of samples assayed.