Human C-Reactive Protein, Pentraxin-Related, CRP ELISA Kit (CKERS-CRP-198H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, other biological fluids
Species Reactivity
Human
Intended Use
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human ADIPOQ in serum, plasma and other biological fluids.
Contents of Kit
1. Human ADIPOQ Microplate: polystyrene microplate coated with a monoclonal antibody against Human ADIPOQ;
2. Standard (freeze dried): 50 ng/mL, 2 vials;
3. Biotin-antibody (100x): 2 vials;
4. HRP-avidin (100x): 2 vials;
5. Reagent Diluent: 25 mL, 2 vials;
6. TMB Substrate: 12 mL, 1 vial;
7. TMB Stop Solution: 1 vial;
8. Wash Buffer (20x): 30 mL, 1 vial;
9. Microtiter plate sealers: 3 sheets.
Storage
Unopened Kit: Store at 2-8°C. Do not use past kit expiration date.
Opened/Reconstituted Reagents: Standard or Sample Diluent; Biotin-antibody Diluent; HRP-avidin Diluent; TMB Substrate; Wash Buffer; TMB Stop Solution. The above mentioned reagents should be stored for up to 1 month at 2-8°C.
Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Sensitivity
Detection Rage: 0.78-50 ng/mL.
Detection Limit: The minimum detectable dose of Human CRP is typically less than 0.2 ng/mL.

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References


The Effect of Mindfulness-Based Stress Reduction Group Counseling on Psychological and Inflammatory Responses of the Women With Breast Cancer

INTEGRATIVE CANCER THERAPIES

Authors: Mirmahmoodi, Motahareh; Mangalian, Parvin; Ahmadi, Atefeh; Dehghan, Mahlagha

Introduction:Breast cancer is common among women and reduces their quality of life. The current study aimed to investigate the effectiveness of mindfulness-based stress reduction group counseling in alleviating psychological responses such as anxiety, depression, stress, and regulating laboratory tests including cortisol and C-reactive protein (CRP) in women with breast cancer.Methods:This randomized clinical trial was conducted based on convenience sampling. Participants were divided into 2 groups (control and intervention groups) with block randomization. The intervention group received mindfulness-based stress reduction group counseling during 8 weeks. The participants completed the Beck anxiety inventory, Beck-II depression inventory, and perceived stress scale before and after the intervention and their blood samples were taken to check their cortisol and CRP.Results:After the intervention, the MBSR group had significantly lower anxiety compared with the control group (P < .001). No significant difference was found between the 2 groups in the reduction of perceived stress and depression (P < .05). In addition, no significant difference was found between the 2 groups in CRP and cortisol levels after the intervention (P > .05).Conclusion:The present study showed the effectiveness of mindfulness-based cognitive therapy in improving anxiety rather than the stress, depression, and inflammatory laboratory factors such as cortisol and CRP in women with breast cancer. Therefore, the psychological symptoms of these patients can be improved at different stages of treatment by providing this type of training.

Successful Clozapine Re-Challenge After Suspected Clozapine-Induced Myocarditis

AMERICAN JOURNAL OF CASE REPORTS

Authors: Hosseini, Seyed Alireza; Skrzypcak, Brianna; Yasaei, Rama; Abdijadid, Sara; Joolhar, Fowrooz; Win, Theingi Tiffany; Moosavi, Leila

Objective: Unusual clinical course Background: Clozapine plays a unique role in the management of treatment-resistant schizophrenia (TRS). Clozapine re-challenge following an episode of myocarditis is controversial, with a very limited literature, although it may be crucial in the recovery of certain patients. To date and to the best of our knowledge, only 10 of 22 studied cases reported successful clozapine retrial after myocarditis. Case Report: We present the case of a 22-year-old Hispanic man with treatment-resistant schizophrenia and poly-substance use disorder (methamphetamine, cannabis, and alcohol) initiated on aggressive clozapine titration after lack of response to several other therapies. Approximately 16 days after clozapine trial, the patient developed cardiac function impairment, presenting with chest pain, notable elevation in several biomarkers (troponin: 0.72 ng/ml, ESR >100 mm/h, CRP: 20.8 mg/dl, and BNP: 999 ng/ml), and a depressed ejection fraction at 25%. Further assessments also showed positive hepatitis A serology. Following discontinuation of clozapine and providing supportive care, the patient's physical symptoms resolved. He had a relapse of psychotic symptoms, which were refractory to treatment with other antipsychotic agents. Subsequently, the patient underwent a second clozapine trial under close monitoring, with resolution of his psychosis. Repeated echocardiography demonstrated improved EF to 50%, transaminitis was resolved, repeat blood test results were normalized, and the patient was discharged while he was stabilized and asymptomatic. Conclusions: This case adds to the previous case reports and suggests that clinicians may consider clozapine re-challenge following an episode of myocarditis based on clinical judgment, on a case-by-case basis, and under close monitoring. We highlight the need for development of clinical guidelines for clozapine re-challenge.

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