Porcine CRP ELISA Kit (DEIA2191)

Regulatory status: For research use only, not for use in diagnostic procedures.

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biological samples
Species Reactivity
Intended Use
The Pig CRP ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay ( ELISA ) for the quantitative determination of C-reactive protein ( CRP ) in pig biological samples.
Contents of Kit
1. Diluent Concentrate
2. Wash Solution Concentrate
3. Enzyme-Antibody Conjugate Concentrate
4. TMB Substrate Solution
5. Stop Solution
6. Microtiter Plate
7. Pig CRP Calibrator
8. Positive Control
Store contents at 4°C.
Detection Range
6.25-200 ng/mL


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Diagnostic Value of C-Reactive Protein in Discrimination between Uncomplicated and Complicated Parapneumonic Effusion


Authors: Kogan, Yana; Sabo, Edmond; Odeh, Majed

Objectives: The role of serum C-reactive protein (CRPs) and pleural fluid CRP (CRPpf) in discriminating uncomplicated parapneumonic effusion (UCPPE) from complicated parapneumonic effusion (CPPE) is yet to be validated since most of the previous studies were on small cohorts and with variable results. The role of CRPs and CRPpf gradient (CRPg) and of their ratio (CRPr) in this discrimination has not been previously reported. The study aims to assess the diagnostic efficacy of CRPs, CRPpf, CRPr, and CRPg in discriminating UCPPE from CPPE in a relatively large cohort. Methods: The study population included 146 patients with PPE, 86 with UCPPE and 60 with CPPE. Levels of CRPs and CRPpf were measured, and the CRPg and CRPr were calculated. The values are presented as mean +/- SD. Results: Mean levels of CRPs, CRPpf, CRPg, and CRPr of the UCPPE group were 145.3 +/- 67.6 mg/L, 58.5 +/- 38.5 mg/L, 86.8 +/- 37.3 mg/L, and 0.39 +/- 0.11, respectively, and for the CPPE group were 302.2 +/- 75.6 mg/L, 112 +/- 65 mg/L, 188.3 +/- 62.3 mg/L, and 0.36 +/- 0.19, respectively. Levels of CRPs, CRPpf, and CRPg were significantly higher in the CPPE than in the UCPPE group (p < 0.0001). No significant difference was found between the two groups for levels of CRPr (p = 0.26). The best cut-off value calculated by the receiver operating characteristic (ROC) analysis for discriminating UCPPE from CPPE was for CRPs, 211.5 mg/L with area under the curve (AUC) = 94% and p < 0.0001, for CRPpf, 90.5 mg/L with AUC = 76.3% and p < 0.0001, and for CRPg, 142 mg/L with AUC = 91% and p < 0.0001. Conclusions: CRPs, CRPpf, and CRPg are strong markers for discrimination between UCPPE and CPPE, while CRPr has no role in this discrimination.

Simulating Strategic Implementation of the CRP to Increase Greater Prairie-Chicken Abundance


Authors: Adkins, Kalysta; Roy, Charlotte L.; Wright, Robert G.; Andersen, David E.

The Conservation Reserve Program (CRP) has the potential to influence the distribution and abundance of grasslands in many agricultural landscapes, and thereby provide habitat for grassland-dependent wildlife. Greater prairie-chickens (Tympanuchus cupido pinnatus) are a grassland-dependent species with large area requirements and have been used as an indicator of grassland ecosystem function; they are also a species of conservation concern across much of their range. Greater prairie-chicken populations respond to the amount and configuration of grasslands and wetlands in agriculturally dominated landscapes, which in turn can be influenced by the CRP; however, CRP enrollments and enrollment caps have declined from previous highs. Therefore, prioritizing CRP reenrollments and new enrollments to achieve the greatest benefit for grassland-dependent wildlife seems prudent. We used models relating either lek density or the number of males at leks to CRP enrollments and the resulting landscape structure to predict changes in greater prairie-chicken abundance related to changes in CRP enrollments. We simulated 3 land-cover scenarios: expiration of existing CRP enrollments, random, small-parcel (4,040 m(2)) addition of CRP grasslands, and strategic, large-parcel (80,000 m(2)) addition of CRP grasslands. Large-parcel additions were the average enrollment size in northwestern Minnesota, USA, within the context of a regional prairie restoration plan. In our simulations of CRP enrollment expirations, the abundance of greater prairie-chickens declined when grassland landscape contiguity declined with loss of CRP enrollments. Simulations of strategic CRP enrollment with large parcels to increase grassland contiguity more often increased greater prairie-chicken abundance than random additions of the same area in small parcels that did not increase grassland contiguity. In some cases, CRP enrollments had no or a negative predicted change in greater prairie-chicken abundance because they provided insufficient grassland contiguity on the landscape, or increased cover-type fragmentation. Predicted greater prairie-chicken abundance increased under large-parcel and small-parcel scenarios of addition of CRP grassland; the greatest increases were associated with large-parcel additions. We suggest that strategic application of the CRP to improve grassland contiguity can benefit greater prairie-chicken populations more than an opportunistic approach lacking consideration of the larger landscape context. Strategic implementation of the CRP can benefit greater prairie-chicken populations in northwestern Minnesota, and likely elsewhere in landscapes where grassland continuity may be a limiting factor. (c) 2020 The Wildlife Society.

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