HIGH SENSITIVITY C-REACTIVE PROTEIN ELISA Kit

Sample

serum

Species Reactivity

Human

Intended Use

For the determination of C-Reactive Protein by enzyme immunoassay in human serum.

Contents of Kit

1. Mouse Anti-CRP Antibody Coated Microwell PlateBreak Apart Wells-Ready To Use.
Contents: One 96 well (12x8) monoclonal antibody-coated microwell plate in a resealable pouch with desiccant.
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.
2. Mouse Anti-CRP Antibody-Horseradish Peroxidase (HRP) Conjugate Concentrate-Requires Preparation.
Contents: Anti-CRP monoclonal antibody-HRP conjugate in a protein-based buffer with a non-mercury preservative.
Volume: 0.3 mL/vial
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.
Preparation: Dilute 1:80 in assay buffer before use (eg. 25 μL of HRP in 2 mL of assay buffer). If the whole plate is to be used dilute 150 μL of HRP in 12 mL of assay buffer. Discard any that is left over.
3. CRP Calibrators-Ready To Use.
Contents: Six vials containing CRP in a protein-based buffer with a non-mercury preservative. Prepared by spiking buffer with a defined quantity of CRP. Calibrated against World Health Organization (WHO) 1st IS 85/506.
*Listed below are approximate concentrations, please refer to vial labels for exact concentrations.

Storage: Refrigerate at 2-8°C
Stability: 12 months in unopened vials or as indicated on label.
Once opened, the calibrators should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles.
4. Controls-Ready To Use.
Contents: Two vials containing CRP in a protein-based buffer with a non-mercury preservative. Prepared by spiking serum with defined quantities of CRP. Refer to vial labels for the acceptable range.
Volume: 0.5 mL/vial
Storage: Refrigerate at 2-8°C
Stability: 12 months in unopened vial or as indicated on label.
Once opened, the controls should be used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles.
5. Wash Buffer Concentrate-Requires Preparation.
Contents: One bottle containing buffer with a non-ionic detergent and a non-mercury preservative.
Volume: 50 mL/bottle
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.
Preparation: Dilute 1:10 in distilled or deionized water before use. If the whole plate is to be used dilute 50 mL of the wash buffer concentrate in 450 mL of water.
6. Assay Buffer-Ready To Use.
Contents: Two vials containing a protein-based buffer with a non-mercury preservative.
Volume: 40 mL/kit
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.
7. TMB Substrate-Ready To Use.
Contents: One bottle containing tetramethylbenzidine and hydrogen peroxide in a non-DMF or DMSO containing buffer.
Volume: 16 mL/bottle
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.
8. Stopping Solution-Ready To Use.
Contents: One vial containing 1M sulfuric acid.
Volume: 6 mL/vial
Storage: Refrigerate at 2-8°C
Stability: 12 months or as indicated on label.

Storage

Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.

General Description

C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in the blood plasma, the levels of which rise in response to inflammation (i.e., C-reactive protein is an acute-phase protein). Its physiological role is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex.
CRP is synthesized by the liver in response to factors released by macrophages and fat cells (adipocytes). It is a member of the pentraxin family of proteins. It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified.