Anti-C Reactive Protein monoclonal antibody, clone C893M

Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting alpha-defensin. In Institute of Cancer Research (ICR) mice, alpha-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that alpha-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of alpha-defensin remains to be determined. In this study, we examined the expression level of messenger RNA (mRNA) encoding six Crp-isoforms and Crp immunoreactivities using singly isolated crypts together with bactericidal activities of Paneth cell secretions from isolated crypts of duodenum, jejunum, and ileum. Here we showed that levels of Crp mRNAs in the single crypt ranged from 5 x 10(3) to 1 x 10(6) copies per 5 ng RNA. For each Crp isoform, the expression level in ileum was 4 to 50 times higher than that in duodenum and jejunum. Furthermore, immunohistochemical analysis of isolated crypts revealed that the average number of Paneth cell per crypt in the small intestine increased from proximal to distal, three to seven-fold, respectively. Both Crp1 and 4 expressed greater in ileal Paneth cells than those in duodenum or jejunum. Bactericidal activities in secretions of ileal Paneth cell exposed to bacteria were significantly higher than those of duodenum or jejunum. In germ-free mice, Crp expression in each site of the small intestine was attenuated and bactericidal activities released by ileal Paneth cells were decreased compared to those in conventional mice. Taken together, Paneth cells and their alpha-defensin in adult mouse appeared to be regulated topographically in innate immunity to control intestinal integrity.

In this paper, an efficient and robust meaningful image encryption (MIE) scheme is developed by combining block compressive sensing (BCS) and singular value decomposition (SVD) embedding. This work devotes to the balanced performance of security, compression, robustness and running efficiency. First, the plain image is divided equally and sparsely represented in discrete cosine transform (DCT) domain, and the coefficient vectors are confused using the coefficient random permutation (CRP) strategy and encrypted into a secret image by compressive sensing. Next, SVD embedding is employed to embed the secret image into a carrier image to create the final meaningful cipher image. In pursuit of superior security, the hyper-chaotic Lorenz system is utilized to generate the updated secret code streams for encryption and embedding with assistance from the counter mode. This scheme is suitable for processing the medium and large images in parallel. Additionally, it exhibits superior robustness and efficiency compared with existing related schemes. Simulation results and comprehensive performance analyses are presented to demonstrate the effectiveness, secrecy and robustness of the proposed scheme. (C) 2020 Elsevier B.V. All rights reserved.