CRP (human) ELISA kit

Sample

Serum or plasma

Species Reactivity

Human

Detection Method

cELISA

Intended Use

The CRP Enzyme-Linked Immunosorbent Assay (ELISA) kit is a colorimetric, competitive immunoassay kit for the quantitative determination of CRP in human serum and plasma. Please read the entire kit insert before performing this assay.

Storage

All components of this kit are stable at 4°C until the kit's expiration date except for the CRP Standard, CRP Antibody, and CRP Conjugate which must be stored at -20°C. Avoid repeated freezethaw cycles.

Precision

The precision numbers listed below represent the percent coefficient of variation for the concentrations of CRP determined in these assays as calculated by a 4 parameter logistic curve fitting program.
Intra-assay precision was determined by taking samples containing low, medium and high concentrations of CRP and running these samples multiple times (n=20) in the same assay.

Inter-assay precision was determined by measuring samples containing low, medium and high concentrations of CRP in multiple assays (n=5) over several days and by different operators.

Sensitivity

The sensitivity or limit of detection of the assay is 8.876 ng/mL, determined by interpolation at 2 standard deviations away from the mean signal of 20 replicates of zero standard.

General Description

C-reactive protein (CRP) is a member of the pentraxin family of proteins, which also includes serum amyloid P component (SAP). Human CRP is comprised of five noncovalently associated subunits and forms a homopentameric structure. It is a widelyused biomarker for systemic inflammation and tissue injury. CRP specifically binds to phosphocholine (PCh) residues of polysaccharides on many microbial pathogens and of apoptotic or necrotic cell membranes in a Ca²⁺ -dependent manner. The PChbound CRP can be recognized by the C1q complex and efficiently initiate the activation of the complement system, which leads to the elimination of foreign pathogens. The binding of CRP to PCh on damaged cells can facilitate the clearance of apoptotic or necrotic host cells, contributing to restoration of normal structure and function of injured tissue. During myocardial infarction and ischemia/reperfusion injury, the same response occurring can lead to additional tissue damage resulting from complement activation. CRP also binds some nuclear constituents which do not contain PCh, such as histones and small nuclear ribonucleoproteins. The binding of CRP to Fc receptors FcγRI and FcγRIIa mediates the interaction of damaged cells or particles with phagocytic cells leading to phagocytosis of the cells or particles. The function of CRP in eliminating foreign pathogens and damaged cells through recruitment of the complement system and phagocytic cells deems CRP a very important molecule in the frontline of innate host defense.
In response to infection, cell damage or tissue injury, the serum CRP concentration may increase by up to 1000 fold. Elevated CRP levels have been reported in patients with infection, chronic inflammatory disorders, myocardial infarction, ischemia/reperfusion injury, atherosclerosis, cancer, pulmonary disorders, metabolic syndrome and depression.

Standard Curve

The results shown below are for illustration only and should not be used to calculate results.

Typical standard curves are shown below. These curves must not be used to calculate CRP concentrations; each user must run a standard curve for each assay.