Rheumatoid Factor IgA ELISA Kit

Name: Rheumatoid Factor IgA ELISA Kit
SKU: DEIA270
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Rheumatoid Factor (RF) IgA ELISA Test Kit has been designed for the the detection and the quantitative determination of RF in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service. This assay is intended for in-vitro diagnostic use only. Laboratory results can never be the only base of a medical report. The patient history and further tests have additionally to be taken into account.

Contents of Kit

1. Microtiter Strips
2. Standards
3. Positive Control
4. Negative Control
5. Enzyme Conjugate
6. Substrate
7. Stop Solution
8. Sample Diluent
9. Washing Buffer
10. Plastic Foils

Storage

1. Store kit components at 2-8°C and do not use after the expiry date on the box outer label.
2. Before use, all components should be allowed to warm up to ambient temperature (18-25°C). After use, the plate should be resealed, the bottle caps replaced and tightened and the kit stored at 2-8°C.
3. The opened kit should be used within three months.

Performance Characteristics

Cross-Reactivity: No cross-reactivity between Rheumatoid factors IgG, IgA and IgM.

Precision

Sensitivity

General Description

Rheumatoid arthritis (RA) is a chronic inflammatory disease of unknown etiology. Rheumatoid arthritis is a systemic disease characterized by chronic proliferation and inflammation of joint cartilage and supporting structures. RA is mainly defined by clinical criteria, in which systematic pathogenetic studies have been hampered by doubts about the presence of common pathogenetic mechanisms and the relative lack of unique laboratory findings. IgG rheumatoid factor has been reported to be present in sera of patients with rheumatoid arthritis both with and without IgM rheumatoid factor activity. Rheumatoid factors are IgA, IgG and IgM immunoglobulins with antibody activity directed against antigenic sites on the Fc portion of IgG molecules. Because of its pentavalent structure and ability to cross-link immunoglobulin G antigen, IgM Rheumatoid Factor is the main class identified by clinically available diagnostic assays for Rheumatoid Factor detection. Rheumatoid factors may exist as the mu, gamma, alpha, and epsilon isotypes. Rheumatoid factors are found in 1 to 4 % of the general population. They are present in 75% of adult patients with the highest incidence of rheumatoid factors occurring in persons over 65 years of age and nearly all patients with Felty and Sjogren syndrome. The clinical correlation of an elevated rheumatoid factor should be interpreted cautiously. Increased titers may accompany a variety of acute immune responses, particularly viral infections and a number of other diseases (e.g., infectious mononucleosis, tuberculosis, leprosy, various parasitic diseases, liver disease, sarcoidosis, and lymphoproliferative syndromes). The earliest tests and those still most widely used rely on the agglutinating properties of the IgM class of rheumatoid factors. Sensitized sheep red blood cell (Waaler-Rose) and latex agglutination tests have been developed and routinely employed. These assays are most sensitive for the detection of Rheumatoid factor that is of the IgM isotype because of its multivalent structure. These tests provide a dilution which is difficult to standardize and have laborious processing and poor reproducibility. In contrast to these assays modern ELISA tests are characterized by a higher sensitivity and by the possibility to differentiate between IgA, IgG and IgM Rheumatoid Factors.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Assessing and mapping landslide susceptibility using different machine learning methods

GEOCARTO INTERNATIONAL

Authors: Orhan, Osman; Bilgilioglu, Suleyman Sefa; Kaya, Zehra; Ozcan, Adem Kursat; Bilgilioglu, Hacer

Abstract

The main aim of the present study was to produce and compare landslide susceptibility maps by using five machine learning techniques, namely, artificial neural network (ANN), logistic regression (LR), support vector machine (SVM), random forest (RF) and, classification and regression tree (CART). The study area was determined as the Arhavi-Kabisre river basin, a region in which the most landslide incidents occur in Turkey. Firstly, a landslide inventory was produced by identifying a total of 252 landslides. Secondly, a total of 11 landslide conditioning factors were considered for the landslide susceptibility mapping. Subsequently, the five machine learning techniques were constructed with the help of the training dataset for the landslide susceptibility maps. Finally, the receiver operating characteristic (ROC), sensitivity, specificity, F-measure, accuracy and kappa index were applied to compare and validate the performance of the five machine learning techniques.

Peanut-like yolk/core-shell MnO/C microspheres for improved lithium storage and the formation mechanism of MnCO3 precursors

JOURNAL OF ALLOYS AND COMPOUNDS

Authors: Chen, Junjie; Yang, Ke; Wang, Jiqi; Zhang, Qiuyu; Zhang, Baoliang

Abstract

The novel peanut-like yolk/core-shell MnO/C microspheres are prepared by combining hydrothermal reaction with precipitation polymerization and calcination technology. The formation mechanism of peanut-like MnCO3 is revealed and the determinant for the morphology of MnO/C microspheres is clarified. It can be obtained yolk-shell and core-shell MnO/C by controlling the thickness of resorcinol-formaldehyde (RF) layers. The electrochemical properties of MnO/C with different structures are evaluated. The enormous volume expansion/contractions of MnO in yolk-shell MnO/C microspheres are accommodated by abundant internal void. The thinner carbon shell provides a shorter path for Li+ diffusion and the Li+ transfer is accelerated. Yolk-shell MnO/C microspheres show high reversible capacity (1073.7 mAh/g after 500 cycles at 1.0 A/g), superior cyclic performance (885.6 mAh/g after 1000 cycles at 2.0 A/g) and rate performance (414.8 mAh/g at 5.0 A/g, 319.6 mAh/g at 10.0 A/g). For core-shell MnO/C microspheres, numerous channels spontaneously formed among MnO secondary particles are conducive to the entry of Li+. It can counteract the Li+ diffusion limitation. Therefore, a stable cyclic performance is maintained. Core-shell MnO/C microspheres exhibited discharge capacities of 712.3 and 514.1 mAh/g at 1.0 and 2.0 A/g after 500 cycles with unconspicuous attenuation. (C) 2020 Elsevier B.V. All rights reserved.