Specificity: Specific for Fc of IgG1, 2, and 4 but not 3
Isotype: IgG
Application: ELISA; Binding Assay
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Summary
Specifications
Antibody Isotype
IgM
Clone
RF-AN
Species Reactivity
Human; Rabbit
Immunogen
The RF-AN cell line, prepared from peripheral blood lymphocytes of an RA patient by transformation with Epstein-Barr virus, secretes an IgM/λ RF
Conjugate
Unconjugated
Applications
Application Notes
Binding Assay: Chimaeric antibody constructs were bound to dansyl-BSA on ELISA plates and RF-AN binding to the immobilised chimaeric antibody detected with enzyme-coupled anti-μ chain specific antibody.
General Notes
The affinity of RF-AN Fab for IgG determined by surface plasmon resonance is very low (K<105 M-1;B.J.S., unpublished data).
Target
Alternative Names
Rheumatoid Factor; RF
Citations
Publication ()
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