Rabbit tPA ELISA kit

Name: Rabbit tPA ELISA kit
SKU: DEIA9389
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

plasma, other biological fluids

Species Reactivity

Rabbit

Detection Method

Sandwich ELISA

Intended Use

The Rabbit tPA ELISA kit is intended for the quantitative determination of active tissue plasminogen activator in rabbit plasma and other biological fluids.

Contents of Kit

1. Avidin Coated Plate: 1-96 well immulon strip plate (8 x 12 removable strips) coated with avidin, blocked, and dried
2. 10X Wash Buffer: 1 bottle of 50 mL wash; bring to 1 x using DI water
3. Biotinylated PAI-1: 1 vial of lyophilized biotinylated PAI-1
4. 10x TBS Buffer pH 7.4: 1 vial of 5 mL TBS buffer
5. Rabbit tPA activity standard: 1 vial of lyophilized standard
6. Anti-rabbit tPA primary antibody: 1 vial of lyophilized polyclonal antibody
7. Anti-sheep secondary antibody: 1 vial of concentrated HRP labeled antibody
8. TMB substrate solution: 1 bottle of 10 mL solution

Storage

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -70°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Detection Range

The assay measures active tPA in the 0.05-20 ng/mL range.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Comparative analysis of eddy current pulsed thermography and long pulse thermography for damage detection in metals and composites

NDT & E INTERNATIONAL

Authors: Wang, Zijun; Zhu, Junzhen; Tian, GuiYun; Ciampa, Francesco

Abstract

Eddy current pulsed thermography (ECPT) and long pulse thermography (LPT) are two emerging non-destructive evaluation techniques that have been recently used for sub-surface defect detection in both metallic and composite components. This paper provides a comparative analysis of these two methods by focussing on the detection of flat bottom holes (FBHs) in both aluminium and carbon fibre reinforced plastic samples. Principal component analysis was used for the post-processing of thermal data in order to enhance the detection of FBHs with various diameters and depths. Results showed that LPT had a better performance for detecting FBHs in low-thermally conductive materials such as carbon fibre-reinforced thermoplastic composites, whilst ECPT revealed a superior performance for detecting FBHs in materials with higher thermal and electrical conductivity such as aluminium.

Aesthetic characteristics of the orthodontic wire with silver plating

BIOMEDICAL RESEARCH-INDIA

Authors: Usui, Takashi; Miyake, Sinjiro; Iwata, Toshio; Otsuka, Takero; Koizumi, So; Shirakawa, Nobukazu; Kawata, Toshitsugu

Abstract

Introduction: The aesthetics of orthodontic appliances represents one of the main concerns of patients receiving orthodontic treatment. A large number of orthodontic appliances have been composed of silver and have a metallic color. The aim of this study is to prepare esthetic orthodontic wires using silver electroplating. The color of the aesthetic wires was assessed using a devised colorimetric measurement method as described later. Materials and methods: A silver-plated wire that we have developed and six brands of esthetic arch wires were assessed. These wires were measured three times using a colorimeter color system 1. Lightness (L*), 2. Hue (a* . b*), 3. Chroma (C*), were evaluated. The colors of these wires need to be compared with the natural tooth color. VITA shade A1 (VA1) as evaluation criteria was measured. Result: 1. In the L* value, the silver-plated wire was the closest to VA1 at 70.5 than (p<0.01). 2. Among the a* values, epoxy coating wire was the closest to VA1 at -1.3. 3. Among the b* and C* values, the silver-plated wire was the most approximate at 8.4 to VA1 (p<0.01). Discussion: The silver-plated wire was closest to the natural tooth color, lightness, chroma, hue. In the future, we are working on development of a more aesthetic wire.