Anti-PLAT monoclonal antibody, clone NM15D16

Eicosanoids are inflammatory signaling lipids that are biosynthesized in response to cellular injury or threat. They were originally thought to be pro-inflammatory molecules, but members of at least one subclass, the lipoxins, are able to resolve inflammation. One step in lipoxin synthesis is the oxygenation of arachidonic acid by 15-lipoxygenase LOX). 15-LOX contains two domains: a Ca2+. binding PLAT domain and a catalytic domain. 15-LOX is a soluble cytosolic protein until binding of Ca2+ to the PLAT domain promotes translocation to the membrane surfac. The role of 15-LOX structural dynamics in this translocation has remained:unclear. We investigated the dynamics of 15-LOX isoform 13 (15-LOX-2) upon binding of Ca2+ and ligands, as well as upon membrane association using hydrogen deuterium exchange mass spectrometry (HDX-MS). We used HDX-MS to probe the solvent accessibility and backbone flexibility of 15-LOX-2, revealing significant differences in deuterium incorporation between the PLAT and catalytic domains, with the PLAT domain demonstrating higher flexibility. Comparison of HDX for 15-LOX-2 in the presence and absence of Ca2+ indicates there are few differences in structural dynamics. Furthermore,,our HDX results involving nanodisc-associated 15-LOX-2 suggest that significant structural and dynamic changes in 15-LOX-2 are not required for membrane association. Our results also show that a substrate lipid binding to the active site in the catalytic domain does induce changes in incorporation of deuterium into the PLAT dornain.-Overall, our results challenge the previous hypothesis that Ca2+ binding induces major structural changes in the PLAT domain and support the hypothesis that is interdorriain communication in 15-LOX-2.

A hybrid photovoltaic thermal (PV/T) solar collector is well-known on its capability to generate electrical and thermal energy, simultaneously. Producing more thermal energy during the process contribute to poor performance on the electrical efficiency of the solar cells. Water and air can help by acting as the heat removal medium. Alternatively, this waste energy can be manipulated to produce hot air/water system. The paper presents the integration of both medium with the conventional PV/T collector. Major components fabricated in the system are two transparent photovoltaic modules connected in parallel as to generate electricity, double pass flat plat air collector, copper water tube and a storage tank as to store hot water produced. Experimental values on solar cell temperature, water temperature and air temperature on both channels have been measured. The performance of the system is calculated based on Hottel-Whillier-Bliss equation. Under controlled indoor environment at radiation level of 800 W/m(2), air flow rate at 0.05 kg/s and water flow rate at 0.02 kg/s; the outlet temperature indicated reading of 27.4 degrees C. This is the best results from the overall experiment. The electrical efficiency achieved was 17% with average electrical power of 145 W and thermal efficiency accomplished was 76%. (C) 2015 Elsevier Ltd. All rights reserved.