G6PD ELISA Kit

Sample

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the quantitative measurement of G6PD in serum, plasma, tissue homogenates, cell culture supernatants, and other biological tissues.

Contents of Kit

1. G6PD Microplate, 96 Wells (12×8 Well strips), -20°C for 6 months
2. G6PD Lyophilized Standard, 2 vials, -20°C for 6 months
3. 100× Biotinylated G6PD Detector Antibody, 1×120 μL, -20°C for 6 months
4. 100× Avidin-HRP Conjugate, 1×120 μL, -20°C for 6 months
5. Sample Diluent, 1×20 mL, -20°C for 6 months
6. Detector Antibody Diluent, 1×12 mL, -20°C for 6 months
7. Conjugate Diluent, 1×12 mL, -20°C for 6 months
8. 25× Wash Buffer, 1×30 mL, Store at 4°C for 6 months
9. Stop Solution, 1×10 mL, Store at 4°C for 6 months
10. TMB Substrate, 1×10 mL, Store at 4°C for 6 months

Storage

Open kit immediately upon receipt. Store components at -20°C (NOTE: exceptions below) for 6 months or until expiration date. Avoid any freeze/thaw cycles.

General Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.