Sample
serum, plasma, cell culture supernatant and other biological samples
Intended Use
In vitro quantitative determination of G6PD concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
0.313-20ng/ml
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C-25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.) The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

Citations
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Yan, L; Elkassar, N; et al. Clonality assays and megakaryocyte culture techniques in essential thrombocythemia. LEUKEMIA & LYMPHOMA 22:31-40(1996).
Dammeyer, P; Arner, ESJ; et al. Human Protein Atlas of redox systems - What can be learnt?. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1810:111-138(2011).