Native Glucose-6-Phosphate Dehydrogenase

Product Overview

One unit of activity is defined as the amount of enzyme that will catalyse the reduction of 1.0 micromole of NAD+ per minute at 25°C under the standard assay method conditions.

Target

Glucose-6-Phosphate Dehydrogenase

Application Notes

This enzyme is useful for enzymatic determination of glucose or ATP when coupled with hexokinase

Format

Lyophilized

Buffer

Enzyme dilution buffer

Storage

Store at -20°C

Introduction

Glucose-6-phosphate dehydrogenase(G6PD or G6PDH) is a cytosolic enzyme in the pentose phosphate pathway (seeimage), a metabolic pathway that supplies reducing energy to cells (such aserythrocytes) by maintaining the level of the co-enzyme nicotinamide adeninedinucleotide phosphate (NADPH). The NADPH in turn maintains the level ofglutathione in these cells that helps protect the red blood cells againstoxidative damage. Of greater quantitative importance is the production ofNADPH for tissues actively engaged in biosynthesis of fatty acids and/orisoprenoids, such as the liver, mammary glands, adipose tissue, and theadrenal glands.

Keywords

Glucose-6-Phosphate Dehydrogenase; G6PD