SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein [His] (DAGC649)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Product Overview
A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations W152C, L452R, D614G) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as CAL.20C or B.1.429 lineage) which emerged in United States.
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 consists of 681 amino acids and predicts a molecular mass of 76.4 kDa. As a result of glycosylation, it migrates as an approximately 93.5 kDa band in SDS-PAGE under reducing conditions.
Alternative Names
SARS-CoV-2 Spike S1; SARS-CoV-2; SARS-CoV-2 S1; SARS-CoV-2 Spike
> 90 % as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
SARS-CoV-2 Spike S1; SARS-CoV-2; SARS-CoV-2 S1; SARS-CoV-2 Spike


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